SKU: 16-0009
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":"246","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant human nucleosomes for enzyme screening assays","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/246/332/Human_recombinant_nucleosome__44913.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=246","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1268","name":"Pack Size","value":"100 μg"},{"id":"1269","name":"Internal Comment","value":"Bulk in LG"},{"id":"1270","name":"Additional Units","value":"Bottom Right of Psylocke"}],"sku":"16-0009","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <p>\n Mononucleosomes, Recombinant Human assembled from recombinant human\n histones expressed in <em>E. coli</em> (two each of histones H2A,\n H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814;\n H3.1-P68431; H4- P62805) wrapped by 147 base pairs of 601 sequence\n DNA. The nucleosome is the basic subunit of chromatin. The 601\n sequence, identified by Lowary and Widom, has high affinity for\n histone octamers and is useful for nucleosome assembly.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16_0009_DNA_web.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0009 DNA Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_DNA_web.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong\n ><br />Mononucleosomes, Recombinant Human run on an agarose\n gel and stained with ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA extracted from nucleosomes\n (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400\n ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16_0009_protein_web.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0009 Protein Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_protein_web.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in Mononucleosomes,\n Human Recombinant (0.5 µg) to demonstrate the purity\n and integrity of the histones in the preparation. Sizes of\n molecular weight markers and positions of the core histones\n (H2A, H2B, H3 and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16_0009_hmtase_web.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\"\n alt=\"16-0009 HMTase Assay\"\n src=\"/content/images/products/nucleosomes/16_0009_hmtase_web.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: HMTase Assay Data </strong><br />\n Mononucleosomes, Recombinant Human used as a substrate in a\n radioactive histone methyltransferase assay with (+SET8) or\n without (-SET8) recombinant SET8, which is an HMTase that\n requires nucleosomal substrates for activity.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"16-0009 DNA Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_DNA_web.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"16-0009 Protein Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_protein_web.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"16-0009 HMTase Assay\"\n src=\"/content/images/products/nucleosomes/16_0009_hmtase_web.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p>\n Purified recombinant mononucleosomes (54.6 µg protein by mass,\n 100 µg protein+DNA in 39.0 µl) in 10 mM Tris-HCl pH 7.5, 1 mM\n EDTA, 25 mM NaCl, 2 mM DTT, 20% glycerol. Nucleosome molecular\n weight = 199,260.8 Da.\n </p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Mononucleosomes, Human Recombinant are highly purified and are\n suitable for use as substrates in enzyme screening assays or for\n nucleosome binding experiments. The absence of post-translational\n histone modifications makes them ideal for conducting enzyme\n activity and screening assays.\n <strong\n >EpiCypher Mononucleosomes, Recombinant do not contain free DNA\n which could alter assayed activities.</strong\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n Lowary & Widom <em>J Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n Luger <em>et al.</em> <em>Methods Mol. Biol.</em> (1999). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/10804500/\"\n target=\"_blank\"\n title=\"Expression and purification of recombinant histones and nucleosome reconstitution\"\n >10804500</a\n ><br />\n <br />\n <strong>Product References:</strong>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrgBfbgAuWF6U5Yt?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0009.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0009 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$425.00","value":425,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Recombinant human nucleosomes for enzyme screening assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":84,"sku":"16-0006","name":"Mononucleosomes, Recombinant Human Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"summary":"\n \n \n \n Description\n \n \n Mononucleosomes ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human Biotinylated"}],"date_added":"3rd Dec 2014","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1251,"name":"Pack Size","value":"50 μg"},{"id":1252,"name":"Internal Comment","value":"Has Bulk - Not Included in Amount"},{"id":1253,"name":"Internal Comment","value":"65 extra units located bottom-right of Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=84","price":{"without_tax":{"currency":"USD","formatted":"$475.00","value":475},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=84"}],"shipping_messages":[],"rating":0,"meta_keywords":"nucleosome, recombinant nucleosome, human nucleosome, chromatin, histone","show_quantity_input":1,"title":"Mononucleosomes, Recombinant Human","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/246/332/Human_recombinant_nucleosome__44913.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human"}]}
Pack Size: 100 μg
Additional Units: Bottom Right of Psylocke
