SKU: 16-0398
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k14ub1-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"877","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant nucleosome with monoubiquitinylated histone H3 at lysine 14 offers a defined substrate for chromatin research and drug discovery. ","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/877/962/nucleosome-recombinant-human-h3k14ub1-dnuc-biotinylated__49438.1645734554.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K14ub1 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=877","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","custom_fields":[{"id":"1127","name":"Pack Size","value":"25 µg"},{"id":"1128","name":"Internal Comment","value":"excess in bottom right of Psylocke"}],"sku":"16-0398","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><em>E. coli</em> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>Biotinylated\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>216,857.3 Da\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n H3K14ub1 designer mononucleosomes (dNucs) consist of 147 base pairs\n of DNA wrapped around an octamer core of histone proteins (two each\n of H2A, H2B, H3.1 and H4) to form a nucleosome, the basic repeating\n unit of chromatin. H3K14ub1 has been shown to promote the activity\n of the methyltransferase Clr4, which methylates H3K9, a known\n repressive mark [1]. The 147 bp 601 sequence, identified by Lowary &\n Widom [2], has high affinity for histone octamers and is useful for\n nucleosome assembly. The DNA in this nucleosome contains a 5’\n biotin-TEG group. H3K14ub1 has a Cys to Ala substitution at position\n 110.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0398-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0398-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0398-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA gel data </strong><br />\n H3K14ub1 dNuc resolved via native PAGE gel and stained with\n ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA (EpiCypher\n <a\n href=\"/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\"\n >18-0005</a\n >; 100 ng). <strong>Lane 2:</strong> Intact H3K14ub1\n nucleosomes (400 ng). <strong>NOTE:</strong> The split band\n for the H3K14ub1 is a gel running artifact of the ubiquitin\n impacting the resolution of the nucleosome.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0398-mass-spec-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0398-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0398-mass-spec-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Mass spec data </strong><br />\n Semi-synthetic H3K14ub1 histone analyzed by high resolution\n mass spectrometry. Expected mass = 23,771.60 Da. Determined\n mass = 23,772.47 Da.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0398-protein-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0398-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0398-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Protein gel data </strong><br />\n Coomassie stained SDS-PAGE gel of proteins in H3K14ub1 dNuc\n (1 µg) demonstrates the purity of histones in the\n preparation. Sizes of molecular weight markers and positions\n of the core histones (H2A, H2B, H3K14ub1 and H4) are\n indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"16-0398-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0398-dna-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0398-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0398-mass-spec-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0398-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0398-protein-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For best\n results, aliquot and avoid freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20%\n glycerol. (14.4 µg protein, 25 µg DNA + protein).\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n H3K14ub1 dNuc is highly purified and suitable for a variety of\n applications, including use as a substrate in enzyme assays,\n high-throughput screening and inhibitor testing, chromatin binding\n studies, protein-protein interaction assays, structural studies, and\n in effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)\n </p>\n <p style=\"margin-bottom: 0\">\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)\n </p>\n <p style=\"margin-bottom: 0\">\n H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)\n </p>\n <p>H4 - P62805</p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Oya et al. <em>EMBO Rep.</em> (2019). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31468675/\"\n title=\"H3K14 ubiquitylation promotes H3K9 methylation for heterochromatin assembly\"\n target=\"new\"\n >\n 31468675</a\n ><br />\n [2] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n target=\"new\"\n >\n 9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0398.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$525.00","value":525,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3K14ub1 Recombinant Nucleosome (dNuc)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[],"related_products":[{"id":677,"sku":"16-0384","name":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27bu-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/677/634/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__94005.1556670077.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/677/634/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__94005.1556670077.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated"}],"date_added":"30th Apr 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":466,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=677","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=677"},{"id":607,"sku":"16-0360","name":"Nucleosome, Recombinant Human, H2AR3me2a dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h2ar3me2a-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" 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Pack Size: 25 µg
