SKU: 18-0005
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=103","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"custom_fields":[{"id":"19","name":"Pack Size","value":"50 μg"}],"customizations":[],"description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 12pt;\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>Nucleosome Assembly 601 Sequence DNA, Biotinylated Description</strong></span></span>:</strong><span style=\"font-family: Arial; font-size: 12pt;\"> Nucleosome Assembly 601 Sequence DNA, Biotinylated is a 147 base-pair double-stranded DNA fragment that was identified by Lowary and Widom (1998) using the SELEX method. The 601 sequence DNA has high affinity for histone octamers and is useful for </span><em style=\"font-family: Arial; font-size: 12pt;\">in vitro</em><span style=\"font-family: Arial; font-size: 12pt;\"> nucleosome assembly. There is a biotin group on the 5' end of the fragment, which makes it ideal for use in nucleosome binding assays and pull-down experiments.</span><br /> <br /> <span style=\"font-family: Arial; font-size: 12pt;\">This product </span><span style=\"font-family: Arial;\">supersedes</span><span style=\"font-family: Arial; font-size: 12pt;\"> Catalog No. 18-0001 as a direct replacement.</span><br /> </td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>Nucleosome Assembly Sequence 601 DNA, Biotinylated</strong></span></span> Formulation:</strong> 50 µg lyophilyzed 601 sequence DNA containing a biotin group on the 5' end.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>Nucleosome Assembly Sequence 601 DNA, Biotinylated Storage and Stability:</strong> Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.</span></span><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><br /> <br /> </span></span></p> </td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong><strong>Nucleosome Assembly Sequence 601 DNA, Biotinylated</strong> Application Notes:</strong> Nucleosome Assembly 601 Sequence DNA, Biotinylated is useful for assembly of nucleosomes using purified or recombinant histone octamers (Catalog No. 16-0005). The biotin group on the DNA allows for pull-down of the nucleosomes for nucleosome binding experiments or enzymatic assays. See website for more information and protocols.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\"> Lowary PT and J Widom (1998). <em>J Mol Biol</em> 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> </span></p> </td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/18-0005.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"18-0005 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Nucleosome core particle reconstitution protocol. <a href=\"https://www.epicypher.com/content/documents/NCP_Reconstitution.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-0005 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/18_0005_web_DNA.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/18_0005_web_DNA.jpg\" alt=\"18-0005 Gel Data\" width=\"241\" height=\"400\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>DNA Gel Data:</strong> Nucleosome Assembly Sequence 601 DNA, Biotinylated (200 ng) run on a 1.8% TBE agarose gel. Migration positions of DNA molecular weight markers are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"103","images":[{"alt":"Nucleosome Assembly 601 Sequence DNA, Biotinylated","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/103/293/DNA__79167.1569012501.gif?c=2"}],"main_image":{"alt":"Nucleosome Assembly 601 Sequence DNA, Biotinylated","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/103/293/DNA__79167.1569012501.gif?c=2"},"max_purchase_quantity":0,"meta_description":"Nucleosome Assembly 601 Sequence DNA, Biotinylated for chromatin assembly and nucleosome reconstitution, useful for pull-down and binding assays","meta_keywords":"nucleosome, mononucleosome, chromatin, chromatin assembly, nucleosome assembly, nuc assembly, mono assembly, histone assembly, histone octamer, histone octamer assembly, octamer, octamer assembly, nucleosome DNA, mononucleosome DNA, 601 sequence, widom 601 sequence, widom 601 sequence,","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"Nucleosome Assembly 601 Sequence DNA, Biotinylated for chromatin assembly and nucleosome reconstitution, useful for pull-down and binding assays","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$115.00","value":115}},"rating":0,"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"18-0005","tags":[],"title":"Nucleosome Assembly 601 Sequence DNA, Biotinylated","upc":"50 µg","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated","warranty":"","weight":"0.01 LBS"}
Pack Size: 50 μg
