Nucleosome

Nucleosome Assembly 601 Sequence DNA, Biotinylated

$115.00
SKU: 18-0005
Pack Size: 50 μg

Description

Nucleosome Assembly 601 Sequence DNA is a 147 base-pair double-stranded DNA fragment that was identified by Lowary and Widom using the SELEX method [1]. The 601 Sequence DNA has high affinity for histone octamers and is useful for in vitro nucleosome assembly. The DNA contains a 5’ biotin-TEG group.

Validation Data

Figure 1: DNA Gel Data
Nucleosome Assembly 601 Sequence DNA, Biotinylated (75 ng) resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Migration positions of DNA molecular weight markers are indicated.

Technical Information

Storage
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
Formulation
50 µg lyophilized 601 Sequence DNA

Application Notes

Nucleosome Assembly 601 Sequence DNA is useful for assembly of nucleosomes using purified or recombinant histone octamers (EpiCypher 16-0001). See Luger et al. (1999) for recommended nucleosome reconstitution protocol. The biotin group on the DNA allows for pull-down of the nucleosomes for nucleosome binding experiments or enzymatic assays.

References

Background References:
[1] Lowary & Widom J. Mol. Biol. (1998). PMID: 9514715
[2] Luger et al. Methods Mol. Biol.. (1999). PMID: 10804500

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