Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

SKU: 16-0396
Pack Size: 25 µg
Nucleosome, Recombinant Human, H2BK120ub dNuc, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H2B (created by a proprietary semi-synthetic method) contains ubiquitin-lysine at position 120. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers, is useful for nucleosome assembly, and contains a 5’ biotin-TEG group.

Nucleosome, Recombinant Human, H2BK120ub dNuc Biotinylated Formulation: Nucleosome, Recombinant Human, H2BK120ub (14.4 μg protein weight, 25 μg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 216,985.9 Da.

Nucleosome, Recombinant Human, H2BK120ub dNuc, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Nucleosome, Recombinant Human, H2BK120ub dNuc, Biotinylated Application Notes: Nucleosome, Recombinant Human, H2BK120ub dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.

References: Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. Luger K et al (1999). Methods Mol Biol 119: 1-16.

View technical datasheet for this product. 16-0396 Datasheet
16-0396 Western Blot Data
Western Blot Data:Western Analysis of Nucleosome, Recombinant Human, H2BK120ub. Top Panel: Unmodified H3 (Lane 1) and H2BK120ub containing nucleosomes (Lane 2) were probed with an anti-H2BK120ub antibody and analyzed via ECL readout. Only the H2BK120ub sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H2BK120ub nucleosomes (Lane 2).
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16-0396 Mass Spec
Mass Spec Data: H2BK120ub protein analyzed by high resolution mass spectrometry. Expected mass = 22,321.8 Da. Determined mass = 22,320.3 Da.
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16-0396 Protein Gel
Protein Gel Data: Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H2BK120ub (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2BK120ub, H3.1, and H4) are indicated.
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16-0396 Native PAGE Data
DNA Gel Data: Nucleosome, Recombinant Human, H2BK120ub dNucs resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact H2BK120ub nucleosome (400 ng).
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