SKU: 16-0390
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=683","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"custom_fields":[{"id":"565","name":"Pack Size","value":"50 µg"}],"customizations":[],"description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><em>E. coli</em> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>Biotinylated\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>199,749.4 Da\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n EpiCypher dNucs™ are semi-synthetic recombinant nucleosomes, containing one or more histone post translational modifications (PTMs). Nucleosomes, the basic repeating unit of chromatin, are the natural targets of chromatin readers and modifying enzymes, providing a physiologically relevant substrate for characterization of epigenetic proteins. dNucs consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.3, and H4). The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3.3K36me3 dNuc contains trimethylated lysine at position 36 on histone H3.3. The DNA in this nucleosome contains a 5’ biotin-TEG group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16-0390-western-blot-data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img alt=\"16-0390-western-blot-data\"\n src=\"/content/images/products/nucleosomes/16-0390-western-blot-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: Western blot data </strong><br />\n Western Analysis of H3.3K36me3 dNuc. <strong>Top Panel:</strong> Unmodified (EpiCypher <a href=\"/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated\">16-0006</a>; Lane 1) and H3.3K36me3 nucleosomes (Lane 2) were probed with an anti-H3.3K36me3 antibody and analyzed via ECL readout. Only the H3.3K36me3 sample produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing unmodified (Lane 1) and H3.3K36me3 nucleosomes (Lane 2).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16-0390-mass-spec-data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img alt=\"16-0390-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0390-mass-spec-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: Mass spec data </strong><br />\n Semi-synthetic H3.3K36me3 histone analyzed by high resolution\n mass spectrometry. Expected mass = 15,238.72 Da. Determined\n mass = 15,237.99 Da.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16-0390-protein-gel-data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img alt=\"16-0390-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0390-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 3: Protein gel data </strong><br />\n Coomassie stained SDS-PAGE gel of proteins in H3.3K36me3 dNuc\n (1 µg) demonstrates the purity of histones in the\n preparation. Sizes of molecular weight markers and positions\n of the core histones (H2A, H2B, H3.3K36me3 and H4) are\n indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16-0390-dna-gel-data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img alt=\"16-0390-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0390-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 4: DNA gel data </strong><br />\n H3.3K36me3 dNuc resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (EpiCypher <a href=\"/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\">18-0005</a>; 100 ng). Lane 2: Intact H3.3K36me3 nucleosomes (400 ng).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img alt=\"16-0390-western-blot-data\"\n src=\"/content/images/products/nucleosomes/16-0390-western-blot-data.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image\" role=\"button\" />\n <img alt=\"16-0390-mass-spec-data\" src=\"/content/images/products/nucleosomes/16-0390-mass-spec-data.jpeg\"\n width=\"200\" class=\"image-picker__side-image image-picker__side-image\" role=\"button\" />\n <img alt=\"16-0390-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0390-protein-gel-data.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image\" role=\"button\" />\n <img alt=\"16-0390-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0390-dna-gel-data.jpeg\"\n width=\"200\" class=\"image-picker__side-image image-picker__side-image\" role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM\n DTT, 20% glycerol (27.4 µg protein, 50 µg DNA + protein)\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n H3.3K36me3 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)\n </p>\n <p style=\"margin-bottom: 0\">\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)\n </p>\n <p style=\"margin-bottom: 0\">\n H3.3 - P84243\n </p>\n <p>H4 - P62805</p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n target=\"new\">\n 9514715</a><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/16-0390.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"683","images":[{"alt":"Nucleosome, Recombinant Human, H3.3K36me3dNuc, Biotinylated","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/683/627/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__26992.1556669776.jpg?c=2"}],"main_image":{"alt":"Nucleosome, Recombinant Human, H3.3K36me3dNuc, 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(dNucs™)"],"custom_fields":[{"id":785,"name":"Pack Size","value":"50 μg"}],"date_added":"26th May 2017","demo":false,"has_options":false,"id":368,"image":{"alt":"Nucleosome, Recombinant Human, H4K20me2 dNuc, Biotinylated","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/368/312/Recombinant_nucleosome__44239.1582226101.png?c=2"},"images":[{"alt":"Nucleosome, Recombinant Human, H4K20me2 dNuc, Biotinylated","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/368/312/Recombinant_nucleosome__44239.1582226101.png?c=2"}],"low_stock_level":null,"name":"Nucleosome, Recombinant Human, H4K20me2 dNuc, Biotinylated","num_reviews":null,"pre_order":false,"price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$575.00","value":575}},"qty_in_cart":0,"rating":null,"show_cart_action":true,"sku":"16-0332","stock_level":null,"summary":" 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Pack Size: 50 µg
