Nucleosome, Recombinant Human, H2AK129ub1 dNuc, Biotinylated

SKU: 16-0400
Pack Size: 25 µg


Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P20671; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H2A (created by a proprietary semi-synthetic method) contains ubiquitin-lysine at position 129. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.

Validation Data

Figure 1: DNA Gel Data
H2AK129ub1 dNuc resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (EpiCypher 18-0005; 100 ng). Lane 2: Intact H2AK129ub1 nucleosomes (400 ng).

Figure 2: Mass Spec Data
Semi-synthetic H2AK129ub1 histone analyzed by high resolution mass spectrometry. Expected mass = 22,551.0 Da. Determined mass = 22,551.05 Da.

Figure 3: Protein Gel Data
Coomassie stained PAGE gel of proteins in H2AK129ub1 dNuc (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2AK129ub1, H2B, H3, and H4) are indicated.

Technical Information

H2AK129ub1 dNuc (14.4 µg protein weight, 25 µg DNA + protein) in 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 216,953.5 Da.
Storage and Stability
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes

H2AK129ub1 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.


Background References:
[1] Lowary & Widom J. Mol. Biol. (1998). PMID: 9514715

Documents & Resources

Current stock: 0