Histone

Histone Octamer, Recombinant Human

$215.00
SKU: 16-0001
Pack Size: 50 μg
  • Species:  Human
  • Source:  E. coli
  • Tag:  None
  • Molecular Weight:  109,536 Da

Description

Human histone octamers (two each of histones H2A, H2B, H3 and H4) are made from recombinant histones expressed in E. coli. Octamers are the protein component of nucleosomes, the basic subunit of chromatin. Assembling this product with DNA bearing a 601 Widom sequence produces a nucleosome (e.g. EpiCypher 18-0005, 18-0006). The 601 Widom sequence, originally discovered by Lowary and Widom [1], has high affinity for octamers. For pre-assembled solutions, see our complete list of nucleosome products.

Validation Data

Figure 1: Protein gel data
Histone Octamer, Recombinant Human (1 µg) run on a SDS-PAGE gel and stained with Coomassie blue to demonstrate the purity of the preparation. The individual histones are indicated.

Figure 2: Methylation assay data
Histone Octamer, Recombinant Human used in methylation assays with GST-tagged SET domains as indicated. Reactions were loaded onto a PAGE gel and subjected to Western blotting. Top panel: anti-H3K9me2. Bottom panel: anti-histone H3 C-term (EpiCypher 13-0001).

Figure 3: Purification data
Chromatogram from gel filtration purification of Histone Octamer, Recombinant Human. Only fractions corresponding to the octamers as indicated were collected and pooled.

Technical Information

Storage
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Formulation
10 mM Tris-HCl pH 7.5, 1 mM EDTA, 2 M NaCl, 2 mM DTT, 20% glycerol

Application Notes

Human recombinant histone octamers can be used for chromatin reconstitution experiments or as substrates for histone modifying enzymes. See Luger et al. (1999) for recommended nucleosome reconstitution protocol. Histone octamers should not be stored under conditions where the salt concentration is lower than 800 mM.

Please see lot-specific Technical Datasheets for product concentration.

Gene & Protein Information

UniProt ID

H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)

H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)

H3.1 - P68431 (alt.names: H3, H3/a, H3/b, H3/c, H3/d)

H4 - P62805

References

Background References:
[1] Lowary & Widom J. Mol. Biol. (1998). PMID: 9514715
[2] Luger et al. Methods Mol. Biol. (1999). PMID: 10804500

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