Account Navigation

Account Navigation

Currency - All prices are in AUD

Currency - All prices are in AUD
EpiCypher

EpiDyne™ Nucleosome Remodeling Substrates
Opening the door for the study of disease-relevant ATP-dependent chromatin remodeling complexes

Nucleosome remodeling regulates DNA access on chromatin and thus impacts gene expression and genome repair. Many ATP-dependent remodeling enzymes are associated with human disease (below) but are challenging targets to study biochemically due to the requirement for nucleosome-based substrates. EpiCypher has addressed this need by developing EpiDyne™, a family of recombinant nucleosome substrates to monitor nucleosome remodeling activity in vitro.

EpiDyne Model

EpiDyne Nucleosome Remodeling Substrates consist of a human histone octamer wrapped with a strong nucleosome positioning sequence (Widom 601) shielding a target motif (purple box). The target motif is either a restriction enzyme recognition site or a DAM methylation sequence. Subsequent to the action of an ATP-dependent remodeler (e.g. RSC or another SWI/SNF ATPase), the target site is exposed, whereupon it can either be cut by a restriction enzyme or methylated by DAM. The latter method is immediately compatible with HTS applications.

Compatible with Multiple Assay Formats:
• Restriction Enzyme Accessibility
• DAM methyltransferase
• HTS Fluorescence-based readouts FRET, FP, etc. (coming soon)

Useful for:
• Inhibitor screening and development
• Structure-Activity Relationship assays
• Biochemical profiling of ATPase family proteins

hSWI/SNF Protein Types of Cancer
hSNF5 (INI1, BAF47, SMARCB1) Malignant rhabdoid tumors, Medulloblastoma, CML Choroid plexus carcinomas
BRG1 (SMARCA4, BAF190, hSNF2) Multiple carcinomas, Prostate cancer, Oral cancers Non-small cell lung cancer
BRM (SMARCA2, BAF190B, SNF2L2) Multiple carcinomas, Non-small cell lung cancer
The mutation or misregulation of human SWI/SNF family ATPases is associated with multiple cancer types.
16-4101 Remodeling Data

ATP-dependent nucleosome remodeling reaction in the presence of DpnII. EpiDyne Nucleosome Remodeling Substrates were incubated for the time indicated with or without (No RSC) the ATP-dependent chromatin remodeler RSC in the presence of ATP (or No ATP as indicated) and the restriction enzyme DpnII. Samples were then resolved on a polyacrylamide gel and stained with ethidium bromide. The red arrow indicates the fragment cleaved from the nucleosomal DNA subsequent to remodeling.

Download the EpiDyne Nucleosome Remodeling Substrate application technical note

Download the EpiDyne Nucleosome Remodeling Substrate brochure


EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1
Cat. No.: 16-4101
Pack Size: 50 μg