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EpiCypher

EpiDyne™ Nucleosome Substrates for Chromatin Remodeling Assays
Opening the door for the study of disease-relevant ATP-dependent chromatin remodeling complexes

Chromatin remodeling regulates DNA access on chromatin and thus impacts gene expression and genome repair. Many ATP-dependent chromatin remodeling enzymes are associated with human disease but are challenging targets to study biochemically due to the requirement for nucleosome-based substrates. EpiCypher has addressed this need by developing EpiDyne, a family of recombinant nucleosome substrates to monitor chromatin remodeling activity in vitro.

Compatible with Multiple Assay Formats:
FRET - NEW!
Restriction Enzyme Accessibility
• DAM methyltransferase

Useful for:
• Inhibitor screening and development
• Structure-Activity Relationship assays
• Biochemical profiling of ATPase family proteins

EpiDyne Restriction Enzyme / MTase Substrate EpiDyne FRET Substrate
EpiDyne Model EpiDyne Model
EpiDyne REA Nucleosome Remodeling Substrate consists of a human histone octamer wrapped with a strong nucleosome positioning sequence (Widom 601) shielding a target motif (purple box). The target motif is either a restriction enzyme recognition site or a DAM methylation sequence. Subsequent to the action of an ATP-dependent remodeler (e.g. RSC or another SWI/SNF ATPase), the target site is exposed, whereupon it can either be cut by a restriction enzyme or methylated by DAM. EpiDyne-FRET Nucleosome Remodeling Substrate consists of a Cy5-labeled human histone octamer (red section of octamer) wrapped by 5' Cy3-labeled DNA (217bp; Cy3 = green ball) with a terminal nucleosome positioning sequence. In its starting state, Cy3-Cy5 FRET is at a maximum. The activity of an ATP-dependent remodeler (e.g. RSC) is detected by a reduction in FRET signal as the Cy3-labeled DNA 5' end is moved away from the Cy5-labeled octamer.
16-4101 Remodeling Data ATP-dependent chromatin remodeling reaction in the presence of DpnII.
16-4101 Remodeling Data Homogeneous ATP-dependent chromatin remodeling reaction read over time.

EpiDyne-FRET Nucleosome Remodeling Substrate application technical note

EpiDyne Restriction Enzyme Assay Nucleosome Remodeling Substrate application technical note

Download the EpiDyne Nucleosome Remodeling Substrate brochure


EpiDyne-FRET Nucleosome / Chromatin Remodeling Assay Substrate NEW!
Cat. No.: 16-4201
Pack Size: 50 μg

EpiDyne Nucleosome / Chromatin Remodeling Assay Substrate ST601-GATC1
Cat. No.: 16-4101
Pack Size: 50 μg

EpiDyne Nucleosome / Chromatin Remodeling Assay Substrate ST601-GATC1, Biotinylated
Cat. No.: 16-4111
Pack Size: 50 μg