HP1β, Recombinant Human, His-Tagged

SKU: 15-0074
Pack Size: 100 µg
  • Type:  Chromodomain
  • Mol. Wgt.:  24.4 kDa
  • Expressed In :  E. coli
  • Epitope Tag:  6xHis


Recombinant human HP1β (CBX1, M31, Heterochromatin protein 1 beta, accession P83916, amino acids 1-184) containing an N-terminal 6xHis tag, expressed in E. coli. HP1β is a component of constitutive (transcriptionally silent) heterochromatin, recruited through the interaction.

Validation Data

Figure 1: Protein gel data
HP1β, Recombinant Human (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

Figure 2: AlphaScreen data
HP1β, Recombinant Human, His-Tagged used in nucleosome binding studies to detect interactions with specific post-translational modifications. The identities of the nucleosomes tested are as follows: rNuc unmodified (black), H3K9me3 (blue).

Technical Information

Recombinant 6xHis-tagged protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM DTT, 5% glycerol.
Storage and Stability
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Application Notes

HP1β, Recombinant Human, His-Tagged is useful for protein binding and screening experiments examining methylated protein and peptide substrates.


Background References:
[1] Li et al. Cell (2014). PMID: 25417107

Product References:

Documents & Resources

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