Mononucleosomes (H3.1 ΔN32), Human Recombinant, Biotinylated

Recombinant mononucleosomes (H3.1ΔN32) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The amino acid sequence of H3.1 begins with glycine 33 (amino acids 1-32 are deleted) and the DNA in this nucleosome contains a 5’ biotin-TEG group.