Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated

SKU: 16-0016
Pack Size: 50 µg
  • Species:  Human
  • Source:  E. coli & synthetic DNA
  • Tag:  Biotinylated
  • Molecular Weight:  193,951.1 Da


Recombinant mononucleosomes (H3.1ΔN32) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The amino acid sequence of H3.1 begins with glycine 33 (amino acids 1-32 are deleted) and the DNA in this nucleosome contains a 5’ biotin-TEG group.

Validation Data

Figure 1: Western blot data
Western analysis of H3.1ΔN32 nucleosome. Top Panel: WT H3.1 (Lane 1) and H3.1ΔN32-containing (Lane 2) nucleosomes were probed with an anti-H3 COOH-terminal antibody and analyzed via ECL readout. Bottom Panel: Detail from Coomassie stained gel showing histones from WT H3.1 (Lane 1) and H3.1ΔN32 nucleosomes (Lane 2). H3.1ΔN32 and H4 co-migrate.

Figure 2: Protein gel data
Coomassie stained SDS-PAGE gel of proteins in H3.1ΔN32 nucleosome (1 μg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.1ΔN32 and H4) are indicated. H3.1ΔN32 and H4 co-migrate.

Figure 3: DNA gel data
H3.1ΔN32 nucleosome resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (EpiCypher 18-0005; 100 ng). Lane 2: Intact H3.1ΔN32 nucleosomes (400 ng).

Technical Information

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, 20% glycerol (27.4 µg protein, 50 µg DNA + protein)

Application Notes

H3.1ΔN32 mononucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. The N-terminal deletion enables study of its role in chromatin biology.

Gene & Protein Information

UniProt ID

H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)

H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)

H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)

H4 - P62805


Background References:
[1] Lowary & Widom J. Mol. Biol. (1998). PMID: 9514715

Documents & Resources

Current stock: 0