Nucleosome, Recombinant Human, H3K4me3 dNuc Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains trimethyl-lysine at position 4. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.
| Nucleosome, Recombinant Human, H3K4me3 dNuc Formulation: Nucleosome, Recombinant Human, H3K4me3 (50 µg total mass, DNA+protein, 27.3 µg protein weight) in 10mM Tris HCl, pH 7.5, 25mM NaCl, 1mM EDTA, 2mM DTT, 20% glycerol. MW = 200,774.
| Nucleosome, Recombinant Human, H3K4me3 dNuc Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
| Nucleosome, Recombinant Human, H3K4me3 dNuc Application Notes: Nucleosome, Recombinant Human, H3K4me3 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K4me3 from EpiCypher does not contain free DNA which could alter assayed activities.
| | References:
| View technical datasheet for this product.  |
|  | Western Blot Data: Western Analysis of Nucleosome, Recombinant Human, H3K4me3. Top Panel: Unmodified H3 (Lane 1) and H3K4me3 containing nucleosomes (Lane 2) were probed with an anti-H3K4me3 antibody and analyzed via ECL readout. Only the H3K4me3 sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing rH3 protein (Lane 1) and H3K4me3 octamer (Lane 2).
(Click image to enlarge) |  | Mass Spec Data: Synthetic H3K4me3 protein analyzed by ESI-TOF mass spectrometry. Expected mass = 15,314.97 Da. Determined mass = 15,314.45 Da.
(Click image to enlarge) |  | Protein Gel Data: Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H3K4me3 (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K4Me3 and H4) are indicated.
(Click image to enlarge) |  | DNA Gel Data: Nucleosome, Recombinant Human, H3K4me3 run on an agarose gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA extracted from nucleosomes (200 ng). Lane 2: Intact nucleosomes (400 ng).
(Click image to enlarge) | |
