Nucleosome, Recombinant Human, H3.1K27me3,S28phos, Biotinylated

Recombinant mononucleosomes (H3.1K27me3,S28phos) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3.1K27me3,S28phos contains trimethyl-lysine at position 27 and phosphoserine at position 28 on histone H3.1. The DNA contains a 5’ biotin-TEG group. H3S28 is an essential residue for modulating PRC2-mediated methylation at H3K27me3 [2].