Tailless Nucleosomes, Recombinant Human, Biotinylated

$545.00
SKU: 16-0027
Pack Size: 50 μg
Tailless Nucleosomes, Human Recombinant, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805). All four of the histones have had their tails enzymatically removed after wrapping by 147 base pairs of 601 positioning sequence DNA. There is a 5’ biotin-TEG group on the DNA which makes these nucleosomes ideal for use as a negative control in binding assays and pull-down experiments. The nucleosome is the basic subunit of chromatin.

Tailless Nucleosomes, Human Recombinant, Biotinylated Formulation: Purified recombinant tailless nucleosomes (50 µg total mass protein+DNA, 25 µg protein) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol.

Tailless Nucleosomes, Human Recombinant, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Tailless Nucleosomes, Human Recombinant, Biotinylated Application Notes: Tailless Nucleosomes, Human Recombinant, Biotinylated are highly purified and are suitable for use as a negative control or substrate in enzyme screening assays or for nucleosome binding experiments. The absence of histone post-translational modifications makes them ideal for conducting enzyme activity and screening assays. The biotin group on the DNA makes pull-down experiments possible, allowing isolation of the nucleosomes after assay completion. EpiCypher Tailless Nucleosomes, Human Recombinant, Biotinylated do not contain free DNA which could alter assayed activities.

References:
Strelow JM et al (2016) J Biomol Screen 21: 786-794. Link
Munari F et al (2012) J Biol Chem 287: 33756-33765. Link

Luger K et al (1999) Methods Mol Biol 119: 1-16. Link
Lowary PT and J Widom (1998) J Mol Biol 276: 19-42. Link
Ausio J et al (1989) J Mol Biol 206(3): 451-63. Link



View technical datasheet for this product. 16-0027 Datasheet

16-0027 SDS-PAGE
Protein Gel Data: Coomassie stained SDS-PAGE to demonstrate purity of the histones in the preparation. Lane 1 shows histone proteins in the nucleosome before enzymatic removal of tails. Lane 2 shows the resulting histone proteins after tail removal. Size of molecular weight markers and positions of the intact core histones (H2A, H2B, H3, and H4) are indicated.
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16-0027 Native PAGE
DNA Gel Data: Recombinant Nucleosomes resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100ng). Lane 2: Intact Nucleosomes (400ng). Lane 3: Tailless Nucleosomes after enzymatic removal of histone tails.
Click image to enlarge.
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