Nucleosome, Recombinant Human, H4R3me2s dNuc, Biotinylated

SKU: 16-0358
Pack Size: 50 μg
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Nucleosome, Recombinant Human, H4R3me2s dNuc, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H4 (created by a proprietary synthetic method) contains symmetric dimethyl-arginine at position 3. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.

Nucleosome, Recombinant Human, H4R3me2s dNuc Biotinylated Formulation: Nucleosome, Recombinant Human, H4R3me2s (27.3 μg protein weight, 50 μg total weight) in μl of 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 199,999.26 Da.

Nucleosome, Recombinant Human, H4R3me2s dNuc, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Nucleosome, Recombinant Human, H4R3me2s dNuc, Biotinylated Application Notes: Nucleosome, Recombinant Human, H4R3me2s dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H4R3me2s dNucs from EpiCypher does not contain free DNA which could alter assayed activities.

References: Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. Luger K et al (1999). Methods Mol Biol 119: 1-16.

View technical datasheet for this product. 16-0358 Datasheet
16-0358 Western Blot
Western Blot Data:Western Analysis of Nucleosome, Recombinant Human, H4R3me2s. Top Panel: Unmodified H4 (Lane 1) and H4R3me2s containing nucleosomes (Lane 2) were probed with an anti-H4R3me2s antibody and analyzed via ECL readout. Only the H4R3me2s sample produced a detectable signal. Bottom Panel: Detailfrom Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H4R3me2s nucleosomes (Lane 2).
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16-0358 MS Data
Mass Spec Data:H4R3me2s protein analyzed by high resolution mass spectrometry. Expected mass = 11,306.15 Da. Determined mass = 11,305.45 Da.
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16-0358 Protein Gel Data
Protein Gel Data: Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H4R3me2s (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4R3me2s) are indicated.
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16-0358 DNA Gel Data
DNA Gel Data: Nucleosome, Recombinant Human, H4R3me2s run on a native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact nucleosomes (400 ng).
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