SKU: 16-0004
{"url":"https://www.epicypher.com/products/nucleosomes/chicken-polynucleosomes-purified","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"101","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Purified oligonucleosomes suitable for enzyme screening and kinetic assays","category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/101/341/Nuc-3B__80947.1516390305.png?c=2","alt":"Chicken Polynucleosomes, Purified"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=101","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1035","name":"Pack Size","value":"50 μg"},{"id":"1036","name":"Internal Comment","value":"Has Bulk in LG"}],"sku":"16-0004","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"><tbody><tr valign=\"top\"><td><table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Oligonucleosomes, Purified Description:</b> Oligonucleosomes purified from chicken erythrocytes. The nucleosome is the basic subunit of chromatin consisting of the histone octamer (two each of the four core histones, H2A, H2B, H3 and H4) wrapped by 147 base pairs of DNA. As shown by the size of the DNA species, Chicken Oligoucleosomes are predominantly hexamers, septamers and octamers.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Oligonucleosomes, Purified Formulation: </b>Purified chicken oligonucleosomes, 100 μg (DNA + protein) in 10 mM Tris-HCl pH 8.0, 1 mM EDTA and 10% glycerol.<b><br /><br /></b></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Oligonucleosomes, Purified Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Oligonucleosomes, Purified Application Notes:</b> Chicken oligonucleosomes are suitable for use in enzyme assays such as acetylation, methylation or phosphorylation, chromatin binding assays, or for use as a positive control in Western blotting.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><b style=\"font-size: 12pt; \">References:</b><br /><span style=\"font-size: 10pt; \">Adhvaryu KK et al (2005). <em>Eukaryot Cell</em> 4: 1455-1564. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/16087750\" target=\"_new\">Link</a><br />Kizer KO et al (2005). <em>Mol Cell Biol</em> 25: 3305-3316. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/15798214\" target=\"_new\">Link</a><br />Morris SA et al (2005). <em>Eukaryot Cel</em>l 4: 1446-1454. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/16087749\" target=\"_new\">Link</a></span><br /><br /></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0004.pdf\" target=\"_blank\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0004 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p></td></tr></tbody></table></td><td><table width=\"300\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16_0004_dna_gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16_0004_dna_gel.jpg\" alt=\"16-0004 DNA Gel\" width=\"200\" /></a></td></tr><tr><td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> DNA was purified from Chicken Oligoucleosomes (10 µg) and run on an agarose gel to show the size of nucleosomal DNA compared to molecular weight markers (base pairs). Note that while mononucleosomal DNA runs at 147 base pairs, oligonucleosomal DNA is roughly 200 base pairs per nucleosome. The migration rates of the various oligonucleosome species (tetramer-4, pentamer-5, <em>etc</em>.) are indicated.<br /><strong>(Click image to enlarge) </strong></span></span></td></tr><tr valign=\"top\"><td valign=\"top\" align=\"center\"><a href=\"/content/images/products/nucleosomes/16_0004_protein_gel.jpg\" target=\"_new\"> <img src=\"/content/images/products/nucleosomes/16_0004_protein_gel.jpg\" alt=\"16-0004 Protein Gel\" width=\"150\" /></a></td></tr><tr><td valign=\"top\" align=\"left\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Chicken Oligonucleosomes (10 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers (kDa) and positions of the core histones (H2A, H2B, H3 and H4) and histone H1 are indicated.<br /><strong>(Click image to enlarge) </strong></span></span></td></tr><tr valign=\"top\"><td valign=\"top\" align=\"center\"><a href=\"/content/images/products/nucleosomes/16_0004_enzyme_gel.jpg\" target=\"_new\"> <img src=\"/content/images/products/nucleosomes/16_0004_enzyme_gel.jpg\" alt=\"16-0004 Protein Gel\" width=\"152\" height=\"125\" /></a></td></tr><tr><td valign=\"top\" align=\"left\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Methylation Assay Data:</strong> Chicken Oligonucleosomes (4 µg) incubated without (-) or with (+) yeast Dot1 enzyme were subjected to Western blotting using and antibody recognizing histone H3 lysine 79 treimethyl (H3 K79me3).<br /><strong>(Click image to enlarge) </strong></span></span></td></tr></tbody></table></td></tr></tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$215.00","value":215,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Purified Chicken Polynucleosomes | Suitable for enzyme screening and kinetic assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":"50 ë_g","options":[],"related_products":[{"id":98,"sku":"16-0003","name":"HeLa Polynucleosomes, Purified","url":"https://www.epicypher.com/products/nucleosomes/hela-polynucleosomes-purified","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"summary":"\n \n \n Species: Human\n \n \n Source: HeLa cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/98/335/Human_recombinant_nucleosome__70596.1516390310.gif?c=2","alt":"HeLa Polynucleosomes, Purified"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/98/335/Human_recombinant_nucleosome__70596.1516390310.gif?c=2","alt":"HeLa Polynucleosomes, Purified"}],"date_added":"3rd Dec 2014","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":873,"name":"Pack Size","value":"50 μg"},{"id":874,"name":"Internal Comment","value":"has bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=98","price":{"without_tax":{"currency":"USD","formatted":"$320.00","value":320},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=98"},{"id":467,"sku":"16-0019","name":"Chicken Mononucleosomes, Purified","url":"https://www.epicypher.com/products/nucleosomes/chicken-mononucleosomes-purified","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"summary":"\n Chicken Mononucleosomes, Purified Description: Mononucleosomes purified from chicken erythro","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/467/340/Nuc-3B__00718.1516390305.png?c=2","alt":"Chicken Mononucleosomes, Purified"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/467/340/Nuc-3B__00718.1516390305.png?c=2","alt":"Chicken Mononucleosomes, Purified"}],"date_added":"26th Sep 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":235,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=467","price":{"without_tax":{"currency":"USD","formatted":"$215.00","value":215},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=467"}],"shipping_messages":[],"rating":0,"meta_keywords":"chicken nucleosome, chicken oligonucleosome, polynucleosome, avian oligonucleosomes, chromatin, octamer, nucleosomes, enzyme assays, acetylation, enzyme screening, enzyme kinetics, methylation, chromatin binding assays, Western blotting, chromatin, epigenetics, 16-0004","show_quantity_input":1,"title":"Chicken Polynucleosomes, Purified","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/101/341/Nuc-3B__80947.1516390305.png?c=2","alt":"Chicken Polynucleosomes, Purified"}]}
Pack Size: 50 μg
