HeLa Polynucleosomes, Purified

SKU: 16-0003
Pack Size: 50 μg
HeLa Polynucleosomes, Purified Description: Human Polynucleosomes purified from HeLa cells. The nucleosome is the basic subunit of chromatin consisting of the histone octamer (two each of the four core histones, H2A, H2B, H3 and H4) wrapped by 147 base pairs of DNA.

HeLa Polynucleosomes, Purified Formulation: Purified HeLa Polynucleosomes at a concentration of (DNA + protein) in 20 mM HEPES, pH 7.5, 1 mM EDTA.

HeLa Polynucleosomes, Purified Storage and Stability: Stable for six months at -80°C from date of receipt. It is recommended to aliquot samples and do not refreeze.

HeLa Polynucleosomes, Purified Application Notes: HeLa Polynucleosomes are suitable for use in enzyme assays such as acetylation or methylation, chromatin binding assays, or for use as a positive control in Western blotting. Use 1-2 µg per reaction.

Background References:
Shi X et al. 2006 Nature 442: 96-99 LINK
Matthews AG et al. 2007 Nature 450: 1106-1110 LINK
Kuo AJ et al 2011 Mol. Cell 44: 609-620 LINK

Product References:
Dickinson BM et al. 2019 bioRxiv LINK
Tian X et al. 2019 Cell 177: 622-638 LINK
Vaughan RM et al. 2019 J Biol. Chem. 294: 15724-15732 LINK

View technical datasheet for this product. 16-0003 Datasheet

16-0003 DNA Gel
DNA Gel Data: DNA was purified from HeLa Poly-nucleosomes, Purified and run on an agarose gel to show the size of nucleosomal DNA compared to molecular weight markers (base pairs).
16-0003 Protein Gel
Protein Gel Data: Coomassie stained PAGE gel of proteins in HeLa Polynucleosomes, Purified to demonstrate the purity of the histones in the preparations. Sizes of molecular weight markers (kDa) and position of the core histones are indicated.
16-0003 Protein Gel
HMTase Assay Data: HeLa Polynucleosomes were used in a radioactive histone methyltransferase assay with recombinant SET8. Radioactive SAM was incubated with HeLa Polynucleosmes (Nucs, 1.5 μg), recombinant SET8 (SET8, 1 μg), or both (SET8+Nucs). HMTase activity co-purifying with the nucleosomes is virtually undetectable.
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