HeLa Mononucleosomes, Purified Description: Human mononucleosomes purified from HeLa cells. The nucleosome is the basic subunit of chromatin consisting of the histone octamer (two each of the four core histones, H2A, H2B, H3 and H4) wrapped by 147 base pairs of DNA.
HeLa Mononucleosomes, Purified Formulation: Purified HeLa mononucleosomes at a concentration of (DNA + protein) in 20 mM HEPES, pH 7.5, 1 mM EDTA.
HeLa Mononucleosomes, Purified Storage and Stability: Stable for six months at -80°C from date of receipt. It is recommended to aliquot samples and do not refreeze.
HeLa Mononucleosomes, Purified Application Notes: HeLa Mononucleosomes are suitable for use in enzyme assays such as acetylation or methylation, chromatin binding assays, or for use as a positive control in Western blotting. Use 1-2 µg per reaction.
HeLa Mononucleosomes, Purified References: Kuo AJ et al (2011). Mol Cell 44: 609-620. Link Matthews AG et al (2007). Nature 450: 1106-1110. Link Shi X et al (2006). Nature 442: 96-99. Link
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DNA Gel Data: DNA was purified from HeLa Mono-nucleosomes, Purified and run on an agarose gel to show the size of nucleosomal DNA compared to molecular weight markers (base pairs).
Protein Gel Data: Coomassie stained PAGE gel of proteins in HeLa Mononucleosomes, Purified to demonstrate the purity of the histones in the preparations. Sizes of molecular weight markers (kDa) and position of the core histones are indicated.
HMTase Assay Data: HeLa Mononucleosomes were used in a radioactive histone methyltransferase assay with recombinant SET8. Radioactive SAM was incubated with HeLa Mononucleosmes (Nucs, 1.5 μg), recombinant SET8 (SET8, 1 μg), or both (SET8+Nucs). HMTase activity co-purifying with the nucleosomes is virtually undetectable.