Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated

SKU: 16-0321
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Nucleosome, Recombinant Human, H3K4me1 dNuc Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethyl-lysine at position 4. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.

* H3.2 K4me1 has a C110A mutation

Nucleosome, Recombinant Human, H3K4me1 dNuc Formulation: Nucleosome, Recombinant Human, H3K4me1 (50 µg total mass, DNA+protein, 27.3 µg protein weight) in 10mM Tris HCl, pH 7.5, 25mM NaCl, 1mM EDTA, 2mM DTT, 20% glycerol. MW = 200,620.

Nucleosome, Recombinant Human, H3K4me1 dNuc Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Nucleosome, Recombinant Human, H3K4me1 dNuc Application Notes: Nucleosome, Recombinant Human, H3K4me1 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.


View technical datasheet for this product. 16-0321 Datasheet
16-0321 Western Blot
Western Blot Data: Western Analysis of Nucleosome, Recombinant Human, H3K4me1. Top Panel: Unmodified H3 (Lane 1) and H3K4me1 containing nucleosomes (Lane 2) were probed with an anti-H3K4me1 antibody and analyzed via ECL readout. Only the H3K4me1 sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing rH3 protein (Lane 1) and H3K4me1 octamer (Lane 2).
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16-0321 MS Data
Mass Spec Data: Synthetic H3K4me1 protein analyzed by ESI-TOF mass spectrometry. Expected mass = 15,238 Da. Determined mass = 15,237.5 Da.
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16-0321 Protein Gel Data
Protein Gel Data: Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H3K4me1 (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K4me1 and H4) are indicated.
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16-0321 Protein Gel Data
DNA Gel Data: Nucleosome, Recombinant Human, H3K4me1 run on an agarose gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA extracted from nucleosomes (200 ng). Lane 2: Intact nucleosomes (400 ng).
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