SKU: 16-2045
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-symmetrically-methylated-199x601-dna-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"794","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant nucleosome with symmetrical CpG methylations on the template DNA offers a defined substrate for chromatin research and drug discovery.","category":["Nucleosomes","Nucleosomes/Methyl DNA Designer Nucleosomes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/794/960/mononucleosomes-recombinant-symmetrically-methylated-199x601-dna-biotinylated__40131.1645734552.jpg?c=2","alt":"Mononucleosomes, Recombinant, Symmetrically Methylated 199x601 DNA, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=794","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"646","name":"Pack size","value":"50 µg"}],"sku":"16-2045","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><em>E. coli</em> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>Biotinylated\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>232,993.9 Da\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n Recombinant mononucleosomes consist of 199 base pairs of DNA wrapped\n around an octamer core of histone proteins (two each of H2A, H2B,\n H3.1, and H4) to form a nucleosome, the basic repeating unit of\n chromatin. The 601 sequence, identified by Lowary and Widom [1], is\n a 147-base pair sequence that has high affinity for histone octamers\n and is useful for nucleosome assembly. The 601 sequence is flanked\n by a symmetrically methylated (bolded/blue) 26 bp sequence\n (underlined) as shown in the DNA sequence below. The DNA in this\n nucleosome contains a 5’ biotin-TEG group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-2045-protein-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-2045-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-2045-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Protein gel data </strong><br />\n Coomassie stained SDS-PAGE of proteins in Mononucleosomes,\n Recombinant, Symmetrically Methylated 199x601 DNA,\n Biotinylated (1 µg) demonstrates the purity of histones in\n the preparation. Molecular weight markers and positions of\n the core histones (H2A, H2B, H3.1 and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-2045-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-2045-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-2045-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: DNA gel data </strong><br />\n Mononucleosomes, Recombinant, Symmetrically Methylated\n 199x601 DNA, Biotinylated resolved via native PAGE gel and\n stained with ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA (100 ng).\n <strong>Lane 2:</strong> Intact nucleosomes (400 ng).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-2045-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-2045-protein-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n alt=\"16-2045-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-2045-dna-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For best\n results, aliquot and avoid freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 mM Tris pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20%\n glycerol. (23.4 µg protein, 50 µg DNA + protein)\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Mononucleosomes, Recombinant, 199x601 DNA, Biotinylated are suitable\n for a variety of applications, including use as a substrate in\n enzyme assays, high-throughput screening and inhibitor testing,\n chromatin binding studies, protein-protein interaction assays,\n structural studies, and in effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">DNA Sequence</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p style=\"line-break: anywhere;\">\n 5’-Bio-TEG\n <u\n >GGACCCTATA<span style=\"color: #4698cb\"><strong>C</strong></span\n >G<span style=\"color: #4698cb\"><strong>C</strong></span\n >GGC<span style=\"color: #4698cb\"><strong>C</strong></span></u\n ><u>GCCGAATTC</u\n >CTGGAGAATCCCGGTCTGCAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCG\n CTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATA\n TACATCCTGT<u\n >GGATCCGC<span style=\"color: #4698cb\"><strong>C</strong></span\n >GGT<span style=\"color: #4698cb\"><strong>C</strong></span\n >G<span style=\"color: #4698cb\"><strong>C</strong></span\n >GAACAGCGACC</u\n >3’\n <br />\n 3’<u\n >CCTGGGATATG<span style=\"color: #4698cb\"><strong>C</strong></span\n >GC<span style=\"color: #4698cb\"><strong>C</strong></span\n >GG<span style=\"color: #4698cb\"><strong>C</strong></span\n >GGCTTAAG</u\n >GACCTCTTAGGGCCAGACGTCCGGCGAGTTAACCAGCATCTGTCGAGATCGTGG\n CGAATTTGCGTGCATGCGCGACAGGGGGCGCAAAATTGGCGGTTCCCCTAATGAGGGATCAGAGGTCCGTGCACAGTCTAT\n ATATGTAGGACA<u\n >CCTAGGCGG<span style=\"color: #4698cb\"><strong>C</strong></span\n >CAG<span style=\"color: #4698cb\"><strong>C</strong></span\n >G<span style=\"color: #4698cb\"><strong>C</strong></span\n >TTGTCGCTGG</u\n >5’\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)\n </p>\n <p style=\"margin-bottom: 0\">\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)\n </p>\n <p style=\"margin-bottom: 0\">\n H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)\n </p>\n <p>H4 - P62805</p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n target=\"new\">\n 9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-2045.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Symmetrically Methylated DNA Recombinant Nucleosome, Biotin","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[{"id":1151,"type":"Configurable_PickList_Set","display_name":"Select Pack Size","required":true,"condition":false,"state":"modifier","values":[{"label":"50 µg","id":81,"data":"50 µg","selected":true}],"partial":"set-radio"}],"related_products":[{"id":791,"sku":"16-2043","name":"Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-hemi-methylated-199x601-dna-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Methyl DNA Designer Nucleosomes"],"summary":"\n Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, Biotinylated Description: Mononuc","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/791/765/Long_tail_hemi-methyl_DNA__92953.1525791282.1280.1280__73777.1603294945.1280.1280__13633.1603295562.gif?c=2","alt":"Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, 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Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-199x601-dna-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Methyl DNA Designer Nucleosomes"],"summary":"\n \n \n Species: Human\n \n \n Source: E. coli & synthetic DNA\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/792/931/mononucleosomes-recombinant-199x601-dna-biotinylated__61382.1645734522.jpg?c=2","alt":"Mononucleosomes, Recombinant, 199x601 DNA, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/792/931/mononucleosomes-recombinant-199x601-dna-biotinylated__61382.1645734522.jpg?c=2","alt":"Mononucleosomes, Recombinant, 199x601 DNA, Biotinylated"}],"date_added":"21st Oct 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ass","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/793/767/Long_tail_non_methyl_DNA__31800.1532696948.1280.1280__64910.1603296544.1280.1280__90181.1606315313.png?c=2","alt":"Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/793/767/Long_tail_non_methyl_DNA__31800.1532696948.1280.1280__64910.1603296544.1280.1280__90181.1606315313.png?c=2","alt":"Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated"}],"date_added":"21st Oct 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1103,"name":"Pack size","value":"50 µg"},{"id":1104,"name":"Internal Comment","value":"21 units of lot 2 in New Lot Rack in Psylocke, included in count"}],"num_reviews":null,"weight":{"formatted":"0.01 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Pack size: 50 µg
