Mononucleosomes (H3.3), Human Recombinant Biotinylated
$475.00
SKU: 16-0011
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-h3-3-human-recombinant-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":"249","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Mononucleosomes (H3.3), Human Recombinant Biotinylated for Enzyme Activity Screening","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)","Nucleosomes/Variant Nucleosomes (vNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/249/329/Histone_variant_nucleosome__25014.1516390310.gif?c=2","alt":"Mononucleosomes (H3.3), Human Recombinant Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=249","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"762","name":"Pack Size","value":"50 μg"},{"id":"763","name":"Internal Comment","value":"Has Bulk - Not Included in Amount"}],"sku":"16-0011","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong><strong style=\"font-family: Arial; font-size: 12pt;\"> Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. There is a 5’ biotin-TEG group on the DNA which makes them ideal for use in nucleosome binding assays and pull-down experiments. The nucleosome is the basic subunit of chromatin. H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e<em>.g.</em> at actively transcribed genes). H3.3-H4 associates <em>in vivo</em> with the HIRA chaperone complex.</span><br /> <br /> <span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Our recombinant nucleosomes are now an even better value than before. Due to our constant efforts to innovate and improve our manufacturing processes, we can now offer more (80% more) of our industry-leading recombinant biotinylated nucleosomes for the same price. </span><br /> <br /> </p></td> </tr> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Purified recombinant mononucleosomes (100 µg total mass, protein+DNA, 54.6 µg protein) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. Nucleosome molecular weight = 200,876 Da.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong><strong> Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong><strong> Application Notes:</strong> Mononucleosomes (H3.3), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. The biotin group on the DNA makes pull-down experiments possible, allowing you to isolate the nucleosomes after your assay is complete. <strong>EpiCypher <strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong> do not contain free DNA which could alter assayed activities.</strong><br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\">Lowary PT and J Widom (1998). <em>J Mol Bio</em>l 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> <span style=\"font-size: 10pt;\">Luger K et al (1999). <em>Methods Mol Biol</em> 119: 1-16. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/10804500\" target=\"_new\">Link</a></span><br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-0011.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-0011 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0011-tds-dna.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0011-tds-dna.jpg\" alt=\"16-0011 DNA Gel\" width=\"281\" height=\"300\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">DNA Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Mononucleosomes (H3.3), Human Recombinant Biotinylated run on an agarose gel and stained with ethidium bromide to visualize DNA. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Lane 1:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Intact nucleosomes (200 ng total, ~100 ng DNA and ~100 ng histone protein) run on gel.<strong style=\"font-family: Arial; font-size: 10pt;\"> Lane 2:</strong> DNA extracted from nucleosomes (400 ng). Intact nucleosomes migrate much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Note absence of free DNA in Lane 1, indicating all DNA complexed with histones.</strong></td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0011-tds-gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0011-tds-gel.jpg\" alt=\"16-0011 Protein Gel\" width=\"225\" height=\"302\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3), Human Recombinant Biotinylated (1 µg) to demonstrate the purity and integrity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. </span></span></td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0011-tds-hmt.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0011-tds-hmt.jpg\" alt=\"16-0011 HMT Data\" width=\"225\" height=\"150\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Histone Methyltransderas Assay Data:</strong> Recombinant SET8 histone methyltransferase was used in radioactive methyltransferase assays with a variety of substrates as indicated, with SET8 exhibiting the highest level of activity in combination with recombinant nucleosomes (16-0011).</span></span></td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Mononucleosomes (H3.3), Human Recombinant Biotinylated for Enzyme Activity Screening","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"nucleosome, h3.3, Mononucleosomes (H3.3), Human Recombinant Biotinylated, 16-0011, chromatin, recombinant chromatin","show_quantity_input":1,"title":"Mononucleosomes (H3.3), Human Recombinant Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/249/329/Histone_variant_nucleosome__25014.1516390310.gif?c=2","alt":"Mononucleosomes (H3.3), Human Recombinant Biotinylated"}]} Pack Size: 50 μg
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-h3-3-human-recombinant-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":"249","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Mononucleosomes (H3.3), Human Recombinant Biotinylated for Enzyme Activity Screening","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)","Nucleosomes/Variant Nucleosomes (vNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/249/329/Histone_variant_nucleosome__25014.1516390310.gif?c=2","alt":"Mononucleosomes (H3.3), Human Recombinant Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=249","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"762","name":"Pack Size","value":"50 μg"},{"id":"763","name":"Internal Comment","value":"Has Bulk - Not Included in Amount"}],"sku":"16-0011","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong><strong style=\"font-family: Arial; font-size: 12pt;\"> Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. There is a 5’ biotin-TEG group on the DNA which makes them ideal for use in nucleosome binding assays and pull-down experiments. The nucleosome is the basic subunit of chromatin. H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e<em>.g.</em> at actively transcribed genes). H3.3-H4 associates <em>in vivo</em> with the HIRA chaperone complex.</span><br /> <br /> <span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Our recombinant nucleosomes are now an even better value than before. Due to our constant efforts to innovate and improve our manufacturing processes, we can now offer more (80% more) of our industry-leading recombinant biotinylated nucleosomes for the same price. </span><br /> <br /> </p></td> </tr> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Purified recombinant mononucleosomes (100 µg total mass, protein+DNA, 54.6 µg protein) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. Nucleosome molecular weight = 200,876 Da.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong><strong> Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong><strong> Application Notes:</strong> Mononucleosomes (H3.3), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. The biotin group on the DNA makes pull-down experiments possible, allowing you to isolate the nucleosomes after your assay is complete. <strong>EpiCypher <strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3), Human Recombinant Biotinylated</strong> do not contain free DNA which could alter assayed activities.</strong><br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\">Lowary PT and J Widom (1998). <em>J Mol Bio</em>l 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> <span style=\"font-size: 10pt;\">Luger K et al (1999). <em>Methods Mol Biol</em> 119: 1-16. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/10804500\" target=\"_new\">Link</a></span><br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-0011.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-0011 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0011-tds-dna.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0011-tds-dna.jpg\" alt=\"16-0011 DNA Gel\" width=\"281\" height=\"300\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">DNA Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Mononucleosomes (H3.3), Human Recombinant Biotinylated run on an agarose gel and stained with ethidium bromide to visualize DNA. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Lane 1:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Intact nucleosomes (200 ng total, ~100 ng DNA and ~100 ng histone protein) run on gel.<strong style=\"font-family: Arial; font-size: 10pt;\"> Lane 2:</strong> DNA extracted from nucleosomes (400 ng). Intact nucleosomes migrate much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Note absence of free DNA in Lane 1, indicating all DNA complexed with histones.</strong></td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0011-tds-gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0011-tds-gel.jpg\" alt=\"16-0011 Protein Gel\" width=\"225\" height=\"302\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3), Human Recombinant Biotinylated (1 µg) to demonstrate the purity and integrity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. </span></span></td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0011-tds-hmt.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0011-tds-hmt.jpg\" alt=\"16-0011 HMT Data\" width=\"225\" height=\"150\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Histone Methyltransderas Assay Data:</strong> Recombinant SET8 histone methyltransferase was used in radioactive methyltransferase assays with a variety of substrates as indicated, with SET8 exhibiting the highest level of activity in combination with recombinant nucleosomes (16-0011).</span></span></td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Mononucleosomes (H3.3), Human Recombinant Biotinylated for Enzyme Activity Screening","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"nucleosome, h3.3, Mononucleosomes (H3.3), Human Recombinant Biotinylated, 16-0011, chromatin, recombinant chromatin","show_quantity_input":1,"title":"Mononucleosomes (H3.3), Human Recombinant Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/249/329/Histone_variant_nucleosome__25014.1516390310.gif?c=2","alt":"Mononucleosomes (H3.3), Human Recombinant Biotinylated"}]} Pack Size: 50 μg
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