SKU: 15-0311
{"AddThisServiceButtonMeta":"","add_this":[{"annotation":"","service":"facebook"},{"annotation":"","service":"email"},{"annotation":"","service":"print"},{"annotation":"","service":"twitter"},{"annotation":"","service":"linkedin"}],"add_to_wishlist_url":"/wishlist.php?action=add&product_id=243","availability":"","bulk_discount_rates":[],"can_purchase":true,"cart_url":"https://www.epicypher.com/cart.php","category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits","Recombinant Proteins/Recombinant Histones"],"custom_fields":[{"id":"121","name":"Pack Size","value":"50 μg"}],"customizations":[],"description":"<div><table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td> <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"> <tbody> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Histone</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b><em>E. coli</em></span></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>29 kDa</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Epitope Tag: </b>by product</span></span> </td> </tr> </tbody> </table> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /> <b>Histone H2A/H2B Dimers, Recombinant Human Description:</b> Histone H2A/H2B dimers made from recombinant histones expressed in <em>E. coli</em> (accession numbers: H2A-P04908; H2B-O60814). Histones H2A and H2B were expressed and purified individually by FPLC, then assembled into dimers that were further purified using gel filtration chromatography. <br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H2A/H2B Dimers, Recombinant Human Formulation:</b> 50 µg of H2A/H2B dimer (0.5 mg/ml) in 10mM Tris-HCl pH 7.5, 2M NaCl, 1mM EDTA, 2mM DTT, 20% glycerol.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H2A/H2B Dimers, Recombinant Human Storage and Stability:</b> Lyophilized proteins are stable for 2 years at -20°C from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. <span class=\"Apple-style-span\" style=\"font-size: 12pt; \"> Stable for 6 months at -80°C <span class=\"Apple-style-span\" style=\"font-size: 12pt; \">after reconstitution.</span></span><br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H2A/H2B Dimers, Recombinant Human Application Notes:</b> Recombinant histone H2A/H2B Dimers are suitable for enzyme assays and nucleosome reconstitution.<br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /> </span></span><br /> </p> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/15-0311.pdf\" target=\"_new\"> <img src=\"/content/documents/tds/icon.png\" alt=\"15-0311 Datasheet\" width=\"30\" height=\"40\" /></a></span></span> </td> </tr> </tbody> </table> </td> <td> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"/content/images/products/proteins/15_0311_gel_tds.jpg\" target=\"_new\"> <img src=\"/content/images/products/proteins/15_0311_gel_tds.jpg\" alt=\"15-0311 Gel and Purity QC Data\" width=\"114\" height=\"250\" /></a> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>QC Protein Data:</strong> Histone H2A/H2B Dimers, Recombinant Human, (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> <tr> <td align=\"center\"><a href=\"/content/images/products/proteins/15_0311_chrom_tds.jpg\" target=\"_new\"> <img src=\"/content/images/products/proteins/15_0311_chrom_tds.jpg\" alt=\"15-0311 Chromatofram Purity QC Data\" width=\"200\"/></a> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>QC Purification Data:</strong> Chromatogram from gel filtration purification of Histone H2A/H2B Dimer, Recombinant Human. Only fractions corresponding to the intact dimer (as indicated, black bar) were collected and pooled.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> </tbody> </table> </td> </tr> </tbody></table> </div>","detail_messages":"","gift_wrapping_available":false,"gtin":null,"id":"243","images":[{"alt":"Histone H2A/H2B Dimer, Recombinant Human","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/243/343/Histone_Octamer__02436.1516390305.gif?c=2"}],"main_image":{"alt":"Histone H2A/H2B Dimer, Recombinant Human","data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/243/343/Histone_Octamer__02436.1516390305.gif?c=2"},"max_purchase_quantity":0,"meta_description":"Histone H2A/H2B Dimer, Recombinant Human Protein for Enzyme Screening Assays","meta_keywords":"histone h2A/H2B tetramer, chromatin, nucleosome, chromatin, H2A, H2B, tetram","min_purchase_quantity":0,"mpn":null,"num_reviews":0,"options":[],"page_title":"Histone H2A/H2B Dimer, Recombinant Human Protein","price":{"tax_label":"Sales Tax","without_tax":{"currency":"USD","formatted":"$165.00","value":165}},"rating":0,"shipping":{"calculated":true},"shipping_messages":[],"show_quantity_input":1,"sku":"15-0311","tags":[],"title":"Histone H2A/H2B Dimer, Recombinant Human","upc":null,"url":"https://www.epicypher.com/products/nucleosomes/histone-h2a-h2b-dimer-recombinant-human","warranty":"","weight":"0.01 LBS"}
Pack Size: 50 μg
