Recombinant BRD4 Bromodomain 1 Description: Recombinant human BRD4 Bromodomain 1 (HUNK1, accession number O60885, amino acids 41 to 180) containing an N-terminal GST tag, expressed in E. coli. BRD4 is a member of the BET sub-family of bromodomain-containing proteins, which also includes BRD2, BRD3 and BRDT. BET proteins have two bromodomains and an ET domain C-terminal to the second bromodomain. BRD4 serves as a cofactor for Myc and is crucial to Myc?s role in acute myeloid leukemia. Inhibition of BRD4 binding suppresses Myc activity and leads to differentiation of AML cells and elimination of AML stem cells.
Recombinant BRD4 Bromodomain 1 Formulation: Recombinant GST-tagged protein at 1 mg/ml in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: Recombinant BRD4 Bromodomain 1 is useful for protein binding and screening experiments examining acetylated protein substrates.
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Protein Gel Data: BRD4 Bromodomain 1 (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)