Asymmetric dimethylation of arginine (ADMA) is a post-translational modification catalyzed by type I arginine methyltransferase enzymes and found on many proteins,including RNA binding proteins and histones. Asym26 recognizes ADMA present at GAR (glycine-arginine rich sequences).
Asym26 antibody is useful for Western blotting (1:500 - 1:2,000 dilution) to detect ADMA on a variety proteins.
Asymmetric Dimethyl-arginine Asym26 References:
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Western Blot Data: Western blot using Asymmetric Dimethyl-arginine Antibody (ADMA) Asym26 antibody (1:500 dilution) on mouse embryonic fibroblast (MEF) whole cell extract derived from cells with (+/+) or without (-/-) the PRMT1 gene. Asym26 detects multiple proteins in the "PRMT1 +/+" cell extract lane, indicating proteins containing ADMA. PRMT1 is responsible for the majority of ADMA in mammals, and thus the majority of Asym26-detectable bands disappear in the PRMT1 null cells. The relative migration positions and molecular weights of protein size standards are indicated, as well as a Western blot recognizing the β-actin protein, a loading control. Detection of ADMA-containing proteins using Asym26 in CARM1 null MEFs is unchanged (data not shown, available upon request). (Click image to enlarge)