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EpiCypher

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated

Cat. No.: 16-4111
List Price: $519.00
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Product Description

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 218 base pair DNA sequence that includes the Widom 601 sequence with an added GATC and a 5' biotin-TEG group.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 243,380 Da.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Application Notes: This product is a substrate for chromatin remodeling assays using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling.
References:


View technical datasheet for this product. 16-4111 Datasheet

View Restiction Enzyme Assay application technical note for this product. 16-4111 Tech Note

16-4111 Protein Gel
DNA Gel Data: Free DNA (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated (Nuc, 370 ng) run on an acrylamide gel and stained with ethidium bromide to visualize DNA. Note absence of free DNA in "Nuc", indicating all DNA complexed with histones.
16-4111 DNA Gel
Protein Gel Data: Coomassie stained PAGE gel of proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated (2 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.
16-4111 Western Blot
Nucleosome Remodeling Data: RSC/ATP-dependent chromatin remodeling reaction in the presence of DpnII restriction enzyme. EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated was incubated with RSC chromatin remodeling complex (10 nm) or without (No RSC) for up to two hours in the presence of 2 mM ATP (or No ATP, as indicated) and 50U of DpnII. Samples were quenched at specified intervals and run on 8% native PAGE gel and stained with ethidium bromide. The apperance of a band at ~200 base pairs (red arrow) indicates cleavage at GATC subsequent to chromatin re remodeling.