SNAP-ChIP™: Sample Normalization and Antibody Profiling for ChIP

SNAP-ChIP uses DNA barcoded recombinant designer nucleosomes (dNucs) as next-generation spike-ins for Chromatin Immunoprecipitation (ChIP). The first product in this family (our K-MetStat, or lysine-methylation status panel) consists of a pool of dNucs carrying fifteen well-studied histone lysine methyl marks and an unmodified control. EpiCypher's K-MetStat panel can easily be added to any ChIP workflow without altering the protocol. However, users can monitor antibody specificity and evaluate technical variability within a ChIP or ChIP-seq experiment for the first time, setting SNAP-ChIP apart from any other spike-in controls (i.e. Drosophila) currently available.

SNAP-ChIP Workflow
SNAP-ChIP K-MetStat Panel
SNAP-ChIP K-MetStat Panel Description: A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431 or...