

CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-me-cut-run-kit-for-dna-methylation-sequencing","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":1367,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"The CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing enables streamlined, high-resolution mapping of DNA methylation at >20-fold reduced sequencing depths compared to whole genome strategies like bisulfite sequencing. ","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1367/1258/me-cut-run-kit-icon__02777.1745245422.jpg?c=2","alt":"CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1367","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1349","name":"Pack Size","value":"24 Reactions"}],"sku":"14-1060-24","description":"<!-- <div class=\"product-general-info\">\n <ul style=\"display: none\" class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"display: none\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div id=\"w-s-3835-14-1048\" style=\"\n width: 240px;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-14-1048-div\">\n <a id=\"bioz-w-pb-14-1048\" style=\"font-size: 12px; color: transparent\" href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a>\n </div>\n </a>\n </li>\n </ul>\n</div> -->\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n The <strong>CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing</strong> enables streamlined,\n high-resolution mapping\n of DNA methylation at >20-fold reduced sequencing depths compared to whole genome strategies like bisulfite\n sequencing. In meCUT&RUN, a GST-tagged MeCP2 methyl binding domain binds methylated DNA, directing the\n selective cleavage and release of DNA methylation-enriched chromatin fragments into solution by an\n immunotethered nuclease (pAG-MNase). Enriched DNA is separated from bead-bound cells, purified, and prepared\n for sequencing by one of two methods: \n </p>\n <p><strong>Option 1</strong> uses a traditional library prep method, such\n as the CUTANA™\n CUT&RUN Library Prep Kit (EpiCypher <a href=\"/products/epigenetics-kits-and-reagents/cutana-cut-run-library-prep-kit\" target=\"_blank\">14-1001/14-1002</a>), to provide ~150 bp resolution profiles of DNA\n methylation enrichment. Only 15-20 million total sequencing reads are required for this strategy, and data are\n analyzed using standard CUT&RUN bioinformatic pipelines. \n </p>\n <p><strong>Option 2</strong> uses a cytosine\n conversion strategy such\n as Enzymatic Methyl-seq (NEB<sup>®</sup> EM-seq™, preferred) or bisulfite sequencing to provide base-pair resolution of\n 5-methylcytosine (5mC). This workflow requires 30-50 million total sequencing reads and is analyzed using standard\n DNA methylation bioinformatic tools; see User Manual for detailed information.\n </p>\n <p>The meCUT&RUN Kit is designed for 8-strip tubes and multi-channel pipetting, enabling a seamless workflow\n that maximizes throughput and reproducibility. The kit is compatible with a variety of inputs including\n cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to\n start with 500,000 cells, comparable data can be generated using as few as 10,000 cells. The low sequencing\n depth requirements, as well as compatibility with diverse sample inputs and low cell numbers,\n make this kit an ideal solution for DNA methylation sequencing.</p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/kits/14-1060-24-dna-fragment-size-distribution-analysis.jpeg\"\n target=\"_blank\" class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"14-1060-24-dna-fragment-size-distribution-analysis\"\n src=\"/content/images/products/kits/14-1060-24-dna-fragment-size-distribution-analysis.jpeg\"\n loading=\"lazy\" class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: meCUT&RUN DNA fragment size distribution\n analysis</strong><br />\n meCUT&RUN was performed as described in <strong>Figure 3</strong>. Library DNA was analyzed by\n Agilent TapeStation<sup>®</sup>. This analysis confirmed that mononucleosomes were predominantly enriched in\n meCUT&RUN (~300 bp peaks represent 150 bp nucleosomes + sequencing adapters).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/kits/14-1060-24-gene-browser-tracks.jpeg\"\n target=\"_blank\" class=\"image-picker__main-image-link\"><img loading=\"lazy\"\n alt=\"14-1060-24-gene-browser-tracks\"\n src=\"/content/images/products/kits/14-1060-24-gene-browser-tracks.jpeg\"\n class=\"image-picker__main-image\" loading=\"lazy\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: Gene browser tracks</strong><br />\n meCUT&RUN was performed as described in <strong>Figure 3</strong>. A 30 kb window at the AJM1 gene\n is shown for\n anti-GST antibody and meCUT&RUN. Tracks are also shown with representative data for meCUT&RUN\n followed by EM-seq (meCUT&RUN-EM) and whole genome EM-seq (WGEM), using the New England Biolabs NEBNext<sup>®</sup> Enzymatic\n Methyl-seq v2 Kit (NEB E8015). The meCUT&RUN kit produced the expected genomic distribution, showing\n enrichment of methylated DNA that approximates the methylated CpG pattern observed in WGEM. Images\n were generated using the Integrative Genomics Viewer (IGV, Broad Institute).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/mecut-run-methods.png?t=1745245682.png\"\n target=\"_blank\" class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"mecut-run-methods\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/mecut-run-methods.png?t=1745245682.png\"\n loading=\"lazy\" class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: meCUT&RUN methods</strong><br />\n meCUT&RUN was performed using the CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing starting with\n 500k K562 cells with either 2.5 µL of GST-MeCP2 (EpiCypher <a href=\"/products/epigenetics-kits-and-reagents/cutana-gst-mecp2-for-me-cut-run\" target=\"_blank\">15-2002</a>) added as the primary binding\n reagent or 0.5 µg of a secondary antibody-only control (anti-GST antibody, EpiCypher <a href=\"/products/epigenetics-kits-and-reagents/cutana-anti-gst-tag-antibody\" target=\"_blank\">13-0073</a>) added\n to determine background cleavage. Library preparation was performed using 5 ng of meCUT&RUN-enriched\n DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit\n (EpiCypher <a href=\"/products/epigenetics-kits-and-reagents/cutana-cut-run-library-prep-kit\" target=\"_blank\">14-1001/14-1002</a>). Libraries were run on an Illumina NextSeq2000 with paired-end\n sequencing (2x50 bp). Sample sequencing depth was 7.7 million reads (anti-GST) and 8.4 million reads\n (GST-MeCP2). Data were aligned to the hg38 genome using Bowtie2. Data were filtered to remove\n duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"14-1060-24-dna-fragment-size-distribution-analysis\"\n src=\"/content/images/products/kits/14-1060-24-dna-fragment-size-distribution-analysis.jpeg\"\n width=\"200\" loading=\"lazy\" class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\" alt=\"14-1060-24-gene-browser-tracks\"\n src=\"/content/images/products/kits/14-1060-24-gene-browser-tracks.jpeg\"\n class=\"image-picker__side-image\" loading=\"lazy\" role=\"button\" />\n <img loading=\"lazy\" alt=\"mecut-run-methods\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/mecut-run-methods.png?t=1745245682.png\"\n loading=\"lazy\" class=\"image-picker__side-image\" role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Kit Contents</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA™ GST-MeCP2 for meCUT&RUN</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-gst-mecp2-for-me-cut-run\" target=\"_blank\">15-2002</a>-04\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Anti-GST Tag Antibody</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-anti-gst-tag-antibody\" target=\"_blank\">13-0073</a>-04\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows\" target=\"_blank\">15-1016</a>-01\n </td>\n </tr>\n\n <tr>\n <td>\n CUTANA™ Concanavalin A Conjugated Paramagnetic Beads\n </td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\" target=\"_blank\">21-1401</a>-01\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ <i>E. coli</i> Spike-in DNA</td>\n <td>\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-e-coli-spike-in-dna\" target=\"_blank\">18-1401</a>-01\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Bead Activation Buffer</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-bead-activation-buffer\" target=\"_blank\">21-1001</a>-01\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Stop Buffer</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-stop-buffer\" target=\"_blank\">21-1003</a>-01\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ 5% Digitonin</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-digitonin\" target=\"_blank\">21-1004</a>-01\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN 8-Strip 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\" target=\"_blank\">10-0009</a>-01\n </td>\n </tr>\n\n <tr>\n <td>Pre-Wash Buffer</td>\n <td>21-1002-01</td>\n </tr>\n\n <tr>\n <td>1 M Spermidine</td>\n <td>21-1005-01</td>\n </tr>\n\n <tr>\n <td>0.5 M EDTA</td>\n <td>21-1006-01</td>\n </tr>\n\n <tr>\n <td>100 mM Calcium Chloride</td>\n <td>21-1007-01</td>\n </tr>\n\n <tr>\n <td>5 M NaCl</td>\n <td>21-1013-04</td>\n </tr>\n\n <tr>\n <td>0.1X TE Buffer</td>\n <td>21-1025-01</td>\n </tr>\n <tr>\n <td>SPRIselect reagent manufactured by Beckman Coulter, Inc.</td>\n <td>21-1405-01</td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN Library Prep Kit</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\" target=\"_blank\">14-1001/14-1002</a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">\n Magnetic Separation Rack, 0.2 mL Tubes\n </td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\" target=\"_blank\">10-0008</a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">\n Magnetic Separation Rack, 1.5 mL Tubes\n </td>\n <td>\n <a href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\" target=\"_blank\">10-0012</a>\n </td>\n </tr>\n <tr>\n <td id=\"specific-row\">CUTANA™ Nuclei Extraction Buffer</td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-nuclei-extraction-buffer\" target=\"_blank\">21-1026</a>\n </td>\n </tr>\n <tr>\n <td id=\"specific-row\">CUTANA™ Protease Inhibitor Tablets</td>\n <td>\n <a href=\"/products/epigenetics-kits-and-reagents/cutana-protease-inhibitor-tablets\" target=\"_blank\">21-1027</a>\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n OPEN KIT IMMEDIATELY and store components at room temperature, 4°C, and -20°C as indicated (see <strong>User Manual corresponding to Kit Version 1</strong>). Stable for 12 months upon date of receipt.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for Use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n See User Manual corresponding to Kit Version 1.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Beckman Coulter, the stylized logo, and SPRIselect are\n trademarks or registered trademarks of Beckman Coulter, Inc.\n in the United States and other countries.\n </p>\n <p>NEB<sup>®</sup>, New England Biolabs<sup>®</sup> and NEBNext<sup>®</sup> are registered trademarks of New England Biolabs, Inc. EM-Seq™ is a trademark of New England Biolabs, Inc.</p>\n </div>\n </div>\n </div>\n\n <h3 style=\"color: #4698cb; font-size: 24px; border-bottom: none\" class=\"custom-title-description\">\n Documents & Resources\n </h3>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <!-- <h3 style=\"color: #4698cb; margin-bottom: 0\">Current Lot</h3> -->\n <h3 style=\"margin-top: 1rem; padding-top: 0\" class=\"sub-title1\">\n Kit Version 1\n </h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/14-1060-24.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet\n </span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a href=\"/content/documents/manuals/me-cut-and-run-kit-manual.pdf\" target=\"_blank\"\n class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">meCUT&RUN Kit Manual</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a href=\"/content/documents/manuals/me-cut-and-run-kit-quick-card.pdf\" target=\"_blank\"\n class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">meCUT&RUN Kit Quick Card</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n \n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\" style=\"outline: none;\">Intellectual Property</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\" style=\"display: none;\">\n <p>\n US Pat. No. 7790379, 11885814 and related patents and pending applications\n </p>\n </div>\n </div>\n </div>\n <!--new-->\n <!-- <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object id=\"wobj-3835-14-1048-q\" type=\"text/html\" data=\"https://www.bioz.com/v_widget_6_0/3835/14-1048/?ex=1\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-14-1048-q-div\" style=\"width: 100%\">\n <a id=\"bioz-w-pb-3835-14-1048-q\" style=\"\n font-size: 12px;\n text-decoration: none;\n color: #4698cb;\n \" href=\"https://www.bioz.com/\" target=\"_blank\"><img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a>\n <a style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \" href=\"https://www.bioz.com/result/14-1048/product/EpiCypher/?cn=14-1048\" target=\"_blank\">\n See more details on Bioz</a>\n </div>\n </div>\n </div>\n </div> -->\n </div>\n</div>\n\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj(\"s\", [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n \"14-1048\",\n \"14-1048\"\n );\n });\n</script>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n }\n\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline white 2px !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n\n .epicypher-table td a {\n text-decoration: underline;\n }\n\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; */\n }\n\n .epicypher-table tr:nth-child(even) {\n background-color: #f9f9f9;\n }\n\n .epicypher-table td:first-child {\n border-left: 1px solid lightgray;\n }\n\n /* Tablet */\n\n @media only screen and (max-width: 1024px) {\n\n /* Force table to not be like tables anymore */\n .epicypher-table th {\n display: none !important;\n }\n\n /* BIOZ */\n td.bioz-w-tooltipx::before {\n display: none;\n }\n\n /* Hide table headers (but not display: none;, for accessibility) */\n .epicypher-table thead tr {\n position: absolute;\n top: -9999px;\n left: -9999px;\n }\n\n .epicypher-table tr {\n border: 1px solid #ccc;\n }\n\n .epicypher-table td {\n /* Behave like a \"row\" */\n /* border: none; */\n border-bottom: 1px solid #eee;\n position: relative;\n padding-left: 30%;\n white-space: inherit;\n }\n\n .epicypher-table td:before {\n /* Now like a table header */\n position: absolute;\n /* Top/left values mimic padding */\n top: 6px;\n left: 6px;\n width: 45%;\n padding-right: 10px;\n white-space: nowrap;\n font-weight: 700;\n }\n\n /*\n Label the data\n */\n .epicypher-table td:nth-of-type(1):before {\n content: \"Item\";\n }\n\n td:nth-of-type(2):before {\n content: \"Cat. 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Description
The CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing enables streamlined, high-resolution mapping of DNA methylation at >20-fold reduced sequencing depths compared to whole genome strategies like bisulfite sequencing. In meCUT&RUN, a GST-tagged MeCP2 methyl binding domain binds methylated DNA, directing the selective cleavage and release of DNA methylation-enriched chromatin fragments into solution by an immunotethered nuclease (pAG-MNase). Enriched DNA is separated from bead-bound cells, purified, and prepared for sequencing by one of two methods:
Option 1 uses a traditional library prep method, such as the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002), to provide ~150 bp resolution profiles of DNA methylation enrichment. Only 15-20 million total sequencing reads are required for this strategy, and data are analyzed using standard CUT&RUN bioinformatic pipelines.
Option 2 uses a cytosine conversion strategy such as Enzymatic Methyl-seq (NEB® EM-seq™, preferred) or bisulfite sequencing to provide base-pair resolution of 5-methylcytosine (5mC). This workflow requires 30-50 million total sequencing reads and is analyzed using standard DNA methylation bioinformatic tools; see User Manual for detailed information.
The meCUT&RUN Kit is designed for 8-strip tubes and multi-channel pipetting, enabling a seamless workflow that maximizes throughput and reproducibility. The kit is compatible with a variety of inputs including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 10,000 cells. The low sequencing depth requirements, as well as compatibility with diverse sample inputs and low cell numbers, make this kit an ideal solution for DNA methylation sequencing.
Validation Data
Figure 1: meCUT&RUN DNA fragment size distribution
analysis
meCUT&RUN was performed as described in Figure 3. Library DNA was analyzed by
Agilent TapeStation®. This analysis confirmed that mononucleosomes were predominantly enriched in
meCUT&RUN (~300 bp peaks represent 150 bp nucleosomes + sequencing adapters).
Figure 2: Gene browser tracks
meCUT&RUN was performed as described in Figure 3. A 30 kb window at the AJM1 gene
is shown for
anti-GST antibody and meCUT&RUN. Tracks are also shown with representative data for meCUT&RUN
followed by EM-seq (meCUT&RUN-EM) and whole genome EM-seq (WGEM), using the New England Biolabs NEBNext® Enzymatic
Methyl-seq v2 Kit (NEB E8015). The meCUT&RUN kit produced the expected genomic distribution, showing
enrichment of methylated DNA that approximates the methylated CpG pattern observed in WGEM. Images
were generated using the Integrative Genomics Viewer (IGV, Broad Institute).
Figure 3: meCUT&RUN methods
meCUT&RUN was performed using the CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing starting with
500k K562 cells with either 2.5 µL of GST-MeCP2 (EpiCypher 15-2002) added as the primary binding
reagent or 0.5 µg of a secondary antibody-only control (anti-GST antibody, EpiCypher 13-0073) added
to determine background cleavage. Library preparation was performed using 5 ng of meCUT&RUN-enriched
DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit
(EpiCypher 14-1001/14-1002). Libraries were run on an Illumina NextSeq2000 with paired-end
sequencing (2x50 bp). Sample sequencing depth was 7.7 million reads (anti-GST) and 8.4 million reads
(GST-MeCP2). Data were aligned to the hg38 genome using Bowtie2. Data were filtered to remove
duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Kit Contents
Item | Cat. No. |
---|---|
CUTANA™ GST-MeCP2 for meCUT&RUN | 15-2002-04 |
CUTANA™ Anti-GST Tag Antibody | 13-0073-04 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows | 15-1016-01 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401-01 |
CUTANA™ E. coli Spike-in DNA | 18-1401-01 |
CUTANA™ Bead Activation Buffer | 21-1001-01 |
CUTANA™ Stop Buffer | 21-1003-01 |
CUTANA™ 5% Digitonin | 21-1004-01 |
CUTANA™ CUT&RUN 8-Strip 0.2 mL Tubes | 10-0009-01 |
Pre-Wash Buffer | 21-1002-01 |
1 M Spermidine | 21-1005-01 |
0.5 M EDTA | 21-1006-01 |
100 mM Calcium Chloride | 21-1007-01 |
5 M NaCl | 21-1013-04 |
0.1X TE Buffer | 21-1025-01 |
SPRIselect reagent manufactured by Beckman Coulter, Inc. | 21-1405-01 |
Recommended Accessory Products
Item | Cat. No. |
---|---|
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
Magnetic Separation Rack, 1.5 mL Tubes | 10-0012 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ Protease Inhibitor Tablets | 21-1027 |
Technical Information
Additional Information
Beckman Coulter, the stylized logo, and SPRIselect are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
NEB®, New England Biolabs® and NEBNext® are registered trademarks of New England Biolabs, Inc. EM-Seq™ is a trademark of New England Biolabs, Inc.
Documents & Resources
Kit Version 1
Intellectual Property
US Pat. No. 7790379, 11885814 and related patents and pending applications