Histone H3K4ac Antibody, SNAP-ChIP® Certified
33 in stock
Powered by BiozThis antibody meets EpiCypher’s “SNAP-ChIP® Certified” criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input). Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody reacts to H3K4ac when present alone as well as in combination with nearby acetylations (tetraAc-H3) and no cross reactivity with other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat panel, is detected.
SNAP-ChIP qPCR Data
Histone H3K4ac antibody (5 μg) was tested in a native ChIP experiment using chromatin from K-562 cells (10 μg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher Catalog No. 19-3001) spiked-in prior to micrococcal nuclease digestion. Specificity (left Y-axis)was determined by qPCR for the DNA barcodes corresponding to modified nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency (right y-axis; log scale) and indicates percentage of the target immunoprecipitated relative to input. tetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac
Luminex Data
Histone H3K4ac antibody was assessed using a Luminex® based approach employing dCypher™ Nucleosome K-AcylStat Panel (EpiCypher Catalog No. 16-9003). The panel comprises biotinylated designer nucleosomes (X-Axis) individually coupled to uniquely identifiable Luminex Magplex® beads. Antibody binding to nucleosomes was tested in multiplex (24-plex) at a 1:1000 dilution, and detected with second layer anti-IgG*PE. Data was generated using a Luminex FlexMAP3D®. Data normalized to relevant on-target (H3K4ac; set to 100) is shown. tetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac
Western Blot Data
Western blot of acid extracts from HeLa cells untreated ( – ) or treated with sodium butyrate ( + ), using EpiCypher Catalog No. 13-0034 at 0.5 µg/mL.
Immunocytochemistry Data
Immunocytochemistry of HeLa cells treated with sodium butyrate, using H3K4ac antibody, EpiCypher Catalog No. 13-0034 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
- Type: Monoclonal
- Mol Wgt.: 15 kDa
- Format: Aff. Pur. IgG
- Host: Rabbit
- Appl.: ChIP, ChIP-Seq, WB, ICC, L
- Reactivity: H, M, WR
Immunogen
A synthetic peptide corresponding to histone H3 acetylated at lysine 4.
Formulation
Protein A affinity-purified recombinant monoclonal antibody (1 mg/mL) in PBS, with 0.09% sodium azide, 1% BSA, and 50% glycerol.
Storage and Stability
Stable for 1 years at -20°C from date of receipt.
- ChIP: 2 – 5 μg per 1×106 cells
- WB: 1 – 2 μg/mL
- ICC: 0.05 – 2 μg/mL
- L: 0.25 – 4 μg/mL
Applications Key:
ChIP-Chromatin IP
E-ELISA
FACS-Flow cytometry
IF-Immunofluorescence
IHC-Immunohistochemistry
ICC-Immunocytochemistry
L-Luminex
IP-Immunoprecipitation
WB-Western Blotting
Reactivity Key:
B-Bovine
Ce-C. elegans
Ch-Chicken
Dm- Drosophila
Eu-Eukaryote
H-Human
M-Mouse
Ma-Mammal
R-Rat
Sc-S.cerevesiae
Sp-S. pombe
WR-Wide Range (predicted)
X-Xenopus
Z-Zebrafish