Symmetric Dimethyl-arginine Antibody (SDMA) Sym10
9 in stock
Powered by BiozSymmetric dimethylation of arginine (sDMA) is a post-translational modification catalyzed by type II arginine methyltransferase enzymes and found on many proteins,including those involved in transcriptional regulation, mRNA splicing, DNA repair and nuclear transport. Sym10 recognizes SDMA present at GAR (glycine-arginine rich sequences).
Western Blot Data
Western blot using Symmetric Dimethyl-arginine Antibody (SDMA) Sym10 (1:500 dilution) on mouse embryonic fibroblast (MEF) whole cell extract derived from cells with (+/+) or without (-/-) the PRMT5 gene. Sym10 detects multiple proteins in the ?PRMT5 +/+? cell extract lane, indicating proteins containing SDMA. PRMT5 is responsible for the majority of SDMA in mammals. The relative migration positions and molecular weights of protein size standards are indicated, as well as a Western blot recognizing the ?-actin protein, a loading control.
- Type: Polyclonal
- Mol Wgt.: N/A
- Format: Serum
- Host: Rabbit
- Appl.: WB
- Reactivity: H, M
Immunogen
KLH-conjugated peptide with the sequence KRGRGRGRG in which arg residues were symmetrically dimethylated.
Formulation
Rabbit serum with 30% glycerol and 0.035% sodium azide.
Application Notes
Sym10 antibody is useful for Western blotting (1:500 – 1:2,000 dilution) to detect SDMA on a variety proteins.
References
Boisvert FM et al (2002) J Cell Biol 159: 957-969. Link
Boisvert FM et al (2003) Mol Cell Proteom 2: 1319-1330. Link
Cëte J et al (2005). J Biol Chem 280: 28476-28483. Link
Deng X et al (2010). PNAS USA 107: 19114?19119. Link
Jung GA et al (2011). Exp Mol Med 43:550-560. Link
