SMARCA5 Chromatin Remodeling Enzyme
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Full length recombinant human SMARCA5 Remodeling Enzyme. SMARCA5 is a core ATPase subunit of the ATP-dependent chromatin remodeling ISWI complexes that regulate nucleosome spacing to control DNA accessibility [1,2]. SMARCA5 containing complexes exhibit substrate preference and distinct remodeling directionalities with different regulatory subunits [3]. Mis-regulation of SMARCA5 is linked with multiple human cancers including leukemia, breast, lung, and gastric cancers [4]. SMARCA5 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.
Figure 1: ATP-dependent chromatin remodeling assay
Figure 2: Protein gel data
- Species: Human
- Source: SF9 cells
- Epitope Tag: FLAG-6His
- Mol Wgt: 123.7 kDa
Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET Substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA5 (see Technical Datasheet), 20 nM EpiDyne®-FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact [email protected].
