SKU: 15-1015
Size/Quantity: 100 reactions

SMARCA2 Chromatin Remodeling Enzyme (Human BRM)

$575.00

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Full length recombinant human SMARCA2 Remodeling Enzyme. SMARCA2 is a core ATPase subunit of the ATP-dependent chromatin remodeling complex SWI/SNF that mobilizes nucleosomes and plays key roles in gene transcription regulation, development, genome replication and repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with more than 20% of human cancers and multiple developmental disorders, making SMARCA2 an emerging candidate therapeutic target [3,4]. SMARCA2 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.

Want to learn more? Check out our EpiDyne® Guide >>

Background References:
[1] Clapier & Carins Annu. Rev. Biochem. (2009). PMID: 19355820
[2] Sundaramoorthy & Owen-Hughes F1000Research (2020). PMID: 32864100
[3] Kadoch & Crabtree Sci. Adv. (2015). PMID: 26601204
[4] Cenik & Shilatifard Nat. Rev (2021). PMID: 32958894

15-1015-chromatin-remodeling-ass
(Click to enlarge)
Figure 1: ATP-dependent chromatin remodeling assay

EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20 nM) incubated with SMARCA2 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

15-1015-protein-gel-data
(Click to enlarge)
Figure 2: Protein gel data
SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.
Path 2 Copy 4
Path 2 Copy 5
  • Species:  Human
  • Source:  SF9 cells
  • Epitope Tag:  6His-FLAG
  • Mol Wgt:  181 kDa

Formulation:
25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin.

 
Storage and Stability:
Stable for six months -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
 

Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA2 (see Technical Datasheet), 20 nM EpiDyne®-FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact [email protected]. 

Gene & Protein Information

  • UniProt ID: P51531-2
  • Gene Name: SMARCA2
  • Protein Name: Probable global transcription activator SNF2L2 
  • Amino Acids: 1-1572 with a C-terminal 6His-FLAG 
  • Alternate Names: BAF190B, BRM, SNF2A, SNF2L2 

Technical Datasheet

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