SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)
112 in stock
Powered by BiozFull length recombinant human SMARCA4 Remodeling Enzyme. SMARCA4 is a core ATPase subunit of the ATP-dependent chromatin remodeling complex SWI/SNF that mobilizes nucleosomes and plays key roles in gene transcription regulation, development, genome replication and repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with more than 20% of human cancers and multiple developmental disorders, making SMARCA4 an emerging candidate therapeutic target [3,4]. SMARCA4 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.
Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20nM) incubated with SMARCA4 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.
Figure 2: Protein gel data
- Species: Human
- Source: SF9 cells
- Epitope Tag: 6xHis-FLAG
- Mol Wgt: 186.6 kDa
25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin.
Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne® -FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA4 (see Technical Datasheet), 20 nM EpiDyne® -FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact [email protected].
Gene & Protein Information
- UniProt ID: P51532-1
- Gene Name: SMARCA4
- Protein Name: Transcription activator BRG1
- Amino Acids: 1-1647 with a C-terminal 6His-FLAG
- Alternate Names: BAF190A, BRG1, SNF2B, SNF2L4
