Mononucleosomes (H3.3 K27M), Recombinant Human, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3.3 is a non-allelic replacement found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution
of M for K at position 27 is a dominant negative inhibitor of the EZH2 methyltransferase complex PRC2 and is associated with pediatric brain cancer (DIPG) [1]. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [2], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.