Fiber-seq
Fiber-seq is a multiomic long-read sequencing (LRS) assay that simultaneously profiles chromatin accessibility, DNA methylation, protein footprints, and genetic variants at single-molecule resolution.
LRS is redefining how researchers study the epigenome by directly capturing genetic and epigenetic features from single DNA molecules. Unlike short-read methods, LRS resolves chromatin features genome-wide, even within highly repetitive and structurally complex genomic regions, while also enabling haplotype phasing. EpiCypher is pioneering complete solutions for long-read sequencing-based epigenomics, starting with Fiber-seq.
Resources:
- Fiber-seq 101: A Multiomic Assay That Goes Beyond ATAC-seq
- CUTANA™ Fiber-seq Protocol
- Fiber-seq Services: Prefer to have our experts run experiments for you? Explore our Fiber-seq services for professional assay support and data delivery.
Shop Fiber-seq products below or Request a Quote to get started.
Fiber-seq Workflow
In the Fiber-seq workflow, isolated nuclei are incubated with CUTANA™ Hia5, an N6-methyladenine methyltransferase (6mA MTase) that selectively methylates adenines within regions of accessible chromatin. After a brief 10 minute labeling step, the reaction is quenched and genomic DNA (gDNA) is purified and prepared for sequencing with direct or native DNA sequencing protocols using either Pacific Biosciences® (PacBio®) HiFi or Oxford Nanopore Technologies® (ONT) Nanopore sequencing platforms. Both long-read sequencing platforms deliver information on 6mA labeling (accessible regions) and endogenous DNA methylation (5mC).
| SKU | Name | Price | hf:tax:product_tag | ||
|---|---|---|---|---|---|
 | 15-1032-8rxn or 15-1032-24rxn | CUTANA™ Hia5 for Fiber-seq | Price range: $495.00 through $1,295.00 | long-read-sequencing new-product | |
 | 21-1026 | CUTANA™ Nuclei Extraction Buffer | $295.00 | accessory-reagents-equipment |