Histone H3.3, Recombinant Human

Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) expressed in E. coli and purified by FPLC. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a nonallelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). H3.3-H4 associates in vivo with the HIRA chaperone complex.