H4S1ar1 Recombinant Nucleosome, Biotinylated

ADP-ribosylation (ADPr) is a reversible histone modification that regulates chromatin structure, transcription, DNA replication, and DNA damage repair [1]. Occurring as either mono- or poly-ADP-ribose, ADPr supports recruitment of DNA repair proteins and chromatin remodelers [1]. Recombinant ADPr nucleosomes provide a defined platform for studying biological, chromatin-based functions of ADP-ribosylation.

Product Highlights:

• Physiologically relevant binding – Nucleosomes provide a physiologically relevant substrate for studying chromatin biology, capturing the multivalent binding environment that many chromatin-associated proteins require.
• Built for versatility – Designed for broad biochemical assays, these nucleosome substrates enable robust, quantitative analysis of protein binding, enzymatic activity, and chromatin interactions in a defined relevant format.
• Research ready performance – Built on deep nucleosome design expertise: EpiCypher’s designer nucleosomes are supported by > 1,000 publications across diverse research applications including immunology, cancer, and molecular biology.

H4S1ar1 (histone H4 serine 1 mono-ADPr) Recombinant Nucleosome, Biotinylated consists of 147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5’ biotin-TEG group and the N-terminus of the H4 histone is acetylated. H4S1ar1 nucleosome contains H4 with mono-ADP-ribosylated serine at position 1.  H4S1ar1 is ADP-ribosylated by poly-ADPr polymerase 1 (PARP1) and PARP2, and ADPr removal is achieved by the glycohydrolase ARH3 [1].