EZH2 CUTANA™ CUT&RUN Antibody
7 in stock
Powered by BiozThis antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. EZH2 catalyzes histone H3 lysine 27 trimethylation (H3K27me3), which leads to transcriptional repression of target genes [1]. EZH2 antibody produces CUT&RUN peaks above background primarily in intronic and intergenic regions (Figure 1) that overlap with H3K27me3 (Figure 2), consistent with its known role as the histone methyltransferase catalytic subunit of the Polycomb Repressive Complex 2 (PRC2).
Trademarks and copyrights
Background References:
[1] Cao et al. Science (2002). PMID: 12351676
CUT&RUN was performed on 500k HeLa cells with 0.5 µg of either EZH2 or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.0 million reads (IgG), 5.1 million reads (EZH2), and 5.7 million reads (H3K27me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: EZH2 peaks in CUT&RUN
Figure 2: EZH2 CUT&RUN representative browser tracks
Figure 3: Immunohistochemistry data
Figure 4: Immunoprecipitation data
Figure 5: Western blot data
- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, IP, WB
- Reactivity: Human, Mouse
- Format: Antigen affinity-purified
- Target Size: 85 kDa
- CUT&RUN: 0.5 µg per reaction
- Immunohistochemistry (IHC): 1:500 – 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Immunoprecipitation (IP): 2 – 10 µg/mg lysate
- Western Blot (WB): 1:500 – 1:2,500
- UniProt ID: Q15910
- Gene Name: enhancer of zeste 2 polycomb repressive complex 2
- Protein Name: Histone-lysine N-methyltransferase EZH2
- Alternate Names: enhancer of zeste homolog 2, ENX1, ENX-1, EZH2b, KMT6, KMT6A, WVS, WVS2
- Target Size: 85 kDa
