EpiDyne-FRET Remodeling Assay Substrate DNA

EpiDyne™-FRET Remodeling Assay Substrate DNA is a 207 base-pair double-stranded Cy3-conjugated DNA fragment. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005), a 5’ Cy3 fluorophore, as well as a 3’ acceptor sequence to accommodate the
histone octamer subsequent to remodeling. The DNA also has a restriction enzyme site embedded in the 601 sequence that is accessible after nucleosome remodeling by the DpnII restriction enzyme.