CUTANA™ Wash Buffer Enhancer Set
In stock
Powered by BiozUpgrade CUT&RUN and CUT&Tag workflows with the CUTANA™ Wash Buffer Enhancer Set, specially formulated to reduce sample clumping and improve bead handling. Designed to integrate seamlessly into existing CUT&RUN and CUT&Tag protocols, the Enhancer Set optimizes wash buffer performance, ensuring more consistent chromatin profiling results. By minimizing bead aggregation, these enhancers promote uniform reagent access, reduce sample loss, and improve overall assay efficiency. Ideal for both low-input and high-throughput applications, this set supports reproducibility and enables greater clarity and reliability in epigenomic experiments.
Key Advantages
- Compatible with CUT&RUN and CUT&Tag – Designed to fit seamlessly into existing CUT&RUN and CUT&Tag protocols without interfering with assay performance.
- Reduces sample clumping & loss – Helps maintain suspension of cells/nuclei and beads, minimizing aggregation and preserving precious samples.
- Improves bead handling – Enhances bead resuspension and enables more efficient magnetic bead separation, allowing for easier pipetting throughout protocol steps.
Increases workflow consistency – Supports more uniform reagent access, reducing technical variability.
Figure 1: CUTANA™ Wash Buffer Enhancer Set improves magnetic bead handling in CUT&Tag
In CUT&Tag, following overnight incubation with primary antibody, tubes were placed on a magnetic rack. Images show tubes before (Panel A) or after (Panel B) removal of supernatant. The sample processed without Wash Buffer Enhancers shows cloudy supernatant, poorly defined pellet boundaries, and increased sample adherence to tube walls, indicating bead aggregation and inefficient magnetic bead separation. The sample with CUTANA™ Wash Buffer Enhancer Set shows sharply defined pellet formation, minimal supernatant cloudiness, and reduced adherence to plastic surfaces, reflecting the improved bead dispersion and handling enabled by the Wash Buffer Enhancers.
EpiCypher recommends including the CUTANA™ Wash Buffer Enhancers (1 and 2, both included in this set) in CUT&RUN and CUT&Tag for optimal assay performance.
CUT&RUN
- If using a CUTANA™ CUT&RUN Kit, follow the Kit User Manual Version 5. Include Wash Buffer Enhancer 1 and Wash Buffer Enhancer 2 each at 1X final concentration in Wash Buffer.
- If using pAG-MNase (EpiCypher 15-1016) and related CUT&RUN products, follow the CUTANA™ CUT&RUN DIY Protocol. Include Wash Buffer Enhancer 1 and Wash Buffer Enhancer 2 each at 1X final concentration in Wash Buffer.
CUT&Tag
- NOTE: CUTANA™ CUT&Tag Kit Version 4 already includes Wash Buffer Enhancers 1 and 2.
- If using a CUTANA™ CUT&Tag Kit (Version 3 or prior), follow the Kit User Manual Version 3. Include Wash Buffer Enhancer 1 and Wash Buffer Enhancer 2 each at 1X final concentration in Wash Buffer 1.
- If using pAG-Tn5 (EpiCypher 15-1017) and related CUT&Tag products, follow the CUTANA™ CUT&Tag DIY Protocol. Include Wash Buffer Enhancer 1 and Wash Buffer Enhancer 2 each at 1X final concentration in Digitonin150 Buffer and Digitonin300 Buffer.
| Item | Cat. No. | Conc | QTY |
|---|---|---|---|
| CUTANA™ Wash Buffer Enhancer 1 | 21-1028 | 1,000X | 100 µL |
| CUTANA™ Wash Buffer Enhancer 2 | 15-1030 | 2,500X | 40 µL |