CUTANA™ 5% Digitonin
162 in stock
Powered by BiozDigitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.
Figure 2: Quantification of cell permeabilization with Digitonin
Quantification of live/dead cells as shown in Figure 1. 0.01% was selected as the optimal concentration of Digitonin to efficiently permeabilize cells.
Figure 1: Cell binding to activated ConA beads
ConA beads were washed with CUTANA™ Bead Activation Buffer and loaded with K562 cells as described in the CUTANA™ CUT&RUN and CUT&Tag protocols (www.epicypher.com/protocols). Trypan Blue staining shows cells before (panels A and B) and after (C and D) bead binding. After incubation with activated beads, the supernatant contains few unbound cells (C), demonstrating efficient binding. Permeabilized cells bound to activated beads are observed in the cell-bead slurry (D).
Storage
Stable for 2 years at -20°C from date of receipt.
Formulation
5% (w/v) digitonin in DMSO.
Instructions for Use
CUTANA™ 5% Digitonin can be used to prepare buffers for use in CUT&RUN or CUT&Tag. If using intact cells, the concentration of digitonin should be optimized for efficient cell permeabilization. To avoid digitonin precipitation and cell lysis, use the minimal concentration of digitonin required for efficient cell permeabilization. EpiCypher has found 0.01% Digitonin to be the optimal concentration for most cell types.
See www.epicypher.com/protocols for detailed protocols. Visit support.epicypher.com for guidance on optimizing digitonin concentration.
| Item | Cat. No. |
|---|---|
| CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
| CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
| CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
| CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
| CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
| CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
| CUTANA™ Nuclei Extraction Buffer | 21-1026 |
| CUTANA™ E. coli Spike-in DNA | 18-1401 |
| Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
| CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
