5x SMARCA Remodeling Assay Buffer
88 in stock
Powered by Bioz5x concentrated reaction buffer for use with SMARCA Remodeling Enzymes (EpiCypher 15-1014 and 15-1015). Optimized for use with SMARCA Remodeling Enzyme, produced in SF9 cells. SMARCA is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.
Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne-FRET Chromatin Remodeling Substrate (20 nM; EpiCypher 16-4201) incubated with SMARCA4 Remodeling Enzyme (EpiCypher 15-1014; concentrations indicated). Curves denote FRET efficiency and chromatin remodeling. Download Technical Datasheet for additional QC Data.
Figure 2: Protein gel data
This product is formulated to perform remodeling reactions using SMARCA Remodeling Enzyme and EpiDyne®-FRET substrate (EpiCypher 16-4201) in a 10 μL reaction containing 20 nM SMARCA, 20 nM EpiDyne-FRET, and 2.5 mM rATP. Enzyme remodels to completion in <30 minutes.
For further information, please contact [email protected] or refer to the EpiDyne®– FRET Technote.
