CUTANA™ Bead Activation Buffer
168 in stock
Powered by BiozCUTANA™ Bead Activation Buffer is used to prepare Concanavalin A (ConA) conjugated paramagnetic beads for CUT&RUN and CUT&Tag. Activated ConA beads are used in both CUT&RUN and CUT&Tag workflows to immobilize cells or nuclei.
Figure 1: Cell binding to activated ConA beads
ConA beads were washed with CUTANA™ Bead Activation Buffer and loaded with K562 cells as described in the CUTANA™ CUT&RUN and CUT&Tag protocols (www.epicypher.com/protocols). Trypan Blue staining shows cells before (panels A and B) and after (C and D) bead binding. After incubation with activated beads, the supernatant contains few unbound cells (C), demonstrating efficient binding. Permeabilized cells bound to activated beads are observed in the cell-bead slurry (D).
Storage
Stable for 12 months at 4°C from date of receipt.
Formulation
20 mM HEPES pH 7.9, 10 mM KCl, 1 mM CaCl2, 1 mM MnCl2.
Instructions for Use
CUTANA™ Bead Activation Buffer includes sufficient buffer for 48 CUT&RUN or CUT&Tag reactions using the CUTANA™ protocols (www.epicypher.com/protocols).
| Item | Cat. No. |
|---|---|
| CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
| CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
| CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
| CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
| CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
| CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
| CUTANA™ Nuclei Extraction Buffer | 21-1026 |
| CUTANA™ E. coli Spike-in DNA | 18-1401 |
| Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
| CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
