SKU: 13-0047 or 13-1047

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag

Price range: $105.00 through $315.00
Quantity SKU Product Price
13-0047Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag - 50 reactions$105.00

In stock

13-1047Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag - 250 reactions$315.00

In stock

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [Kaya-Okur, 2019]. For best results, use with CUTANA pAG-Tn5 (EpiCypher 15-1017 / 15-1117).

*Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies. 

CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of either IgG (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), or H3K27me3 (ThermoFisher MA5-11198) antibodies, followed by 0.5 µg of anti-rabbit secondary antibody for CUTANA™ CUT&Tag. The EpiCypher Direct-to-PCR CUT&Tag protocol was used. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 5.0 million reads (IgG), 6.2 million reads (H3K4me3), and 7.2 million reads (H3K27me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.

  • Type:  Mixed Monoclonal*
  • Target Size:  N/A
  • Format:  Affinity Purified IgG
  • Host:  Goat
  • Reactivity:  Anti-Rabbit
  • Applications:  CUT&Tag 
Formulation
Protein G affinity-purified antibody in PBS pH 7.2, with 0.09% sodium azide.
 
Storage and Stability
Store at 4°C short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles.
 
Immunogen
Recombinant full-length rabbit immunoglobulin protein.

CUT&Tag: 0.5 µg per reaction

Technical Datasheet