Heterotypic [H3 • H3K14ac] Recombinant Nucleosome, Biotinylated

Heterotypic [H3 • H3K14ac] Recombinant Nucleosome, Biotinylated is a fully defined semi-synthetic nucleosome containing
one unmodified histone H3 and one histone H3 with acetylated lysine at position 14. [H3 • H3K14ac] nucleosome consists of
147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.2,
and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5’ biotin-TEG group, and histone
H3.2 has a Cys to Ala substitution at position 110. In heterotypic [H3 • H3K14ac] nucleosomes, the single acetylated histone
H3 tail interacts with the SAGA HAT complex to promote acetylation of the unmodified sister histone tail [1].