Tailless Recombinant Nucleosome with Linker DNA, Biotinylated

Recombinant mononucleosomes consist of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’ biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence [1] flanked by 26 bp linker sequences as underlined below. After assembly, histone tails were enzymatically removed. This product is ideal for use as a negative control in binding assays and pull-down experiments.