EpiDyne-FRET Nucleosome Remodeling Assay Substrate

This product provides a high throughput fluorescent readout of nucleosome remodeling when paired with enzymatically active
remodeling complexes, such as SMARCA4, SMARCA2, and ACF (EpiCypher 15-1014, 15-1015, 15-1013). The EpiDyne®-FRET mononucleosome is assembled from recombinant human histones expressed in E. coli (two each of histones H2A-Cy5, H2B, H3.2 and H4; accession numbers: H2A-P04908; H2BO60814; H3.2-Q71DI3; H4-P62805) wrapped with a 212 base-pair DNA template bearing a 5’ Cy3, the Widom 601 nucleosome positioning element [1], and a DpnII restriction enzyme motif (GATC) that is exposed upon remodeling. H2A has a Thr to Cys substitution at residue 120, where Cy5 is conjugated. H3.2 has a Cys to Ala substitution at residue 110.