SKU: 16-3104
Size/Quantity: 25 µg

Dinucleosomes, Recombinant Human, Non-Biotinylated

$425.00

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Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support.

Dinucleosomes are comprised of two recombinant octamers (each containing two copies of histone proteins H2A, H2B, H3.1, and H4) positioned on a single, 442 base pair DNA template. Mononucleosomes are the basic repeating unit of chromatin; however, a dinucleosome substrate enables studying longer range chromatin interactions. The DNA contains two 601 positioning sequences (underlined, below) separated by a 60 base pair linker. The 601 sequence, identified by Lowary & Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly.

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16-3104-protein-gel-data
Figure 1: Protein gel data
Coomassie stained SDS-PAGE gel of proteins in Dinucleosomes (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.
16-3104-dna-gel-data
Figure 2: DNA gel data
Dinucleosomes resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact dinucleosomes (400 ng).
Path 2 Copy 4
Path 2 Copy 5
Kit Contents
Storage
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Recommended Accessory Reagents

Technical Datasheet