Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Non-Biotinylated

Recombinant mononucleosomes (H3Cit2,8,17) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3Cit2,8,17 dNuc contains citrulline (instead of arginine) at positions 2, 8, and 17 on histone H3.2 and a Cys to Ala substitution at position 110.