Nucleosome, Recombinant Human, H3.1S28phos dNuc, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,
H2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains phosphoserine at position 28. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.