Nucleosome, Recombinant Human, H3K27cr dNuc, Biotinylated

H3K27cr designer mononucleosomes (dNucs™) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3K27cr dNuc contains crotonyl-lysine at position 27 on histone H3.2. The DNA contains a 5’ biotin-TEG group. H3K27cr has a Cys to Ala substitution at position 110.